Daidzein alleviated the pathologies in intestinal tissue against ischemia-reperfusion

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dc.contributor.authorDurgun, C.
dc.contributor.authorAsir, F.
dc.date.accessioned2024-04-24T17:33:27Z
dc.date.available2024-04-24T17:33:27Z
dc.date.issued2023
dc.departmentDicle Üniversitesien_US
dc.description.abstract- OBJECTIVE: The aim of this study was to investigate the effect of daidzein against intestinal ischemia-reperfusion injury in rats.MATERIALS AND METHODS: Thirty male Wistar albino rats with a mean weight of 200-250 gr were used. Animals were categorized into sh-am, ischemia-reperfusion (IR), and IR+Daidzein group. 3-hour ischemia of intestine was created by occluding superior mesenteric artery and then left for 3-hour reperfusion. In IR+daidze-in group, after ischemia, 50 mg/kg daidzein was orally administered to the animals. Blood sam-les were collected for biochemical assays. Intestine tissues were excised for histopathologic and immunohistochemical processing.RESULTS: Malondialdehyde (MDA) increased, and Catalase (CAT) and Glutathione (GSH) de-creased after IR in intestine tissue. Daidzein treatment decreased MDA and increased CAT and GSH level in IR+Daidzein group. Histopathologically, sham group showed normal intestinal tissue histology. In IR group, epithelial and villi degeneration, edema, leukocyte infiltration, vascular dilatation and congestion was observed. After Daidzein treatment, these pathologies were improved. The caspase-6 expression was mainly negative in sham group. After IR, caspase-6 reaction was very high in IR group. Daidzein reduced caspase-6 expression in IR+-Daidzein group. Ki67 immune staining was negative in the sham group. In IR group, Ki67 ex-pression was increased in inflammatory cells, deep glandular cells and in some goblet cell nuclei. In IR+ Daidzein group, Ki67 expression was decreased due to reduced inflammation.CONCLUSIONS: IR injury causes oxidative stress, apoptosis and inflammation. Daidzein treatment improved histopathology against intestinal IR.en_US
dc.identifier.endpage1493en_US
dc.identifier.issn1128-3602
dc.identifier.issue4en_US
dc.identifier.pmid36876688
dc.identifier.scopus2-s2.0-85149584327
dc.identifier.scopusqualityQ2
dc.identifier.startpage1487en_US
dc.identifier.urihttps://hdl.handle.net/11468/20690
dc.identifier.volume27en_US
dc.identifier.wosWOS:001006037300005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherVerduci Publisheren_US
dc.relation.ispartofEuropean Review For Medical and Pharmacological Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectDaidzeinen_US
dc.subjectCaspase-6en_US
dc.subjectKi-67en_US
dc.titleDaidzein alleviated the pathologies in intestinal tissue against ischemia-reperfusionen_US
dc.titleDaidzein alleviated the pathologies in intestinal tissue against ischemia-reperfusion
dc.typeArticleen_US

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