Preparation of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for insulin recognition

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dc.contributor.authorCavus, Aynur
dc.contributor.authorBaysal, Zubeyde
dc.contributor.authorAlkan, Huseyin
dc.date.accessioned2024-04-24T16:11:06Z
dc.date.available2024-04-24T16:11:06Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn the present study, affinity adsorption technique was studied for insulin adsorption. Firstly, insulin-imprinted supermacroporous cryogel was prepared for the insulin adsorption. N-methacryloyl-(L)-histidine methyl ester (MAH) was chosen as the monomer. Insulin was complexed with MAH, and insulin-imprinted p(HEMA MAH) [insulin-(MIP)] cryogel was prepared by free radical polymerization with 2-hydroxyethyl methacrylate (HEMA), N,N,N',N'-tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) in an ice bath. Then, insulin was removed from the cryogel by using 0.1 M glycine-HCl buffer (pH: 3.5). The characterization of the cryogel was carried out by using scanning electron microscopy (SEM) and swelling test. The equilibrium swelling ratios of the cryogels were found to be 8.56 +/- 0.42 g H2O/g polymer for p(HEMA) and 7.20 +/- 0.36 g H2O/g polymer for insulin-p(HEMA MAH). Insulin adsorption experiments were performed under different conditions, such as flow rate, medium pH, initial insulin concentration and ionic strength. It was observed that insulin could be repeatedly adsorbed and desorbed with MIP cryogel without any significant decrease in the adsorption capacity. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipDicle University Scientific Research Foundation [DUBAP-09-FF-70]en_US
dc.description.sponsorshipThis work was supported by Dicle University Scientific Research Foundation (DUBAP-09-FF-70).en_US
dc.identifier.doi10.1016/j.colsurfb.2013.01.075
dc.identifier.endpage89en_US
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid23466546
dc.identifier.scopus2-s2.0-84874697261
dc.identifier.scopusqualityQ1
dc.identifier.startpage84en_US
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2013.01.075
dc.identifier.urihttps://hdl.handle.net/11468/15269
dc.identifier.volume107en_US
dc.identifier.wosWOS:000318141900011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofColloids and Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectInsulinen_US
dc.subjectAffinity Adsorptionen_US
dc.subjectMolecular Recognitionen_US
dc.subjectMolecular Imprintingen_US
dc.subjectCryogelen_US
dc.titlePreparation of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for insulin recognitionen_US
dc.titlePreparation of poly(hydroxyethyl methacrylate) cryogels containing L-histidine for insulin recognition
dc.typeArticleen_US

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