A comparative study of bioassays based on enzyme biosynthesis in Escherichia coli and Bacillus subtilis exposed to heavy metals and organic pesticides

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dc.contributor.authorGuven, K
dc.contributor.authorTogrul, S
dc.contributor.authorUyar, F
dc.contributor.authorOzant, S
dc.contributor.authorDe Pomerai, DI
dc.date.accessioned2024-04-24T16:18:44Z
dc.date.available2024-04-24T16:18:44Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractTwo enzyme assays, namely beta-galactosidase in Escherichia coli (E.C.3.2.1.23) and alpha-amylase in Bacillus subtilis (E.C.3.2.1.1), were compared following exposure to various concentrations of heavy metals and organic pesticides. It was shown that alpha-amylase biosynthesis in the Gram-positive bacteria was more sensitive to heavy metals, particularly to mercury as low as 0.1 ppm, compared to beta-galactosidase in the Gram-negative bacteria. The results of organic pesticide treatment were more clear-cm when comparing both assays. Alpha-amylase test system can detect the inhibition by the organometallic fungicides Maneb and Mancozeb at as low as 0.1 ppm, while no inhibition with beta-galactosidase system up to as high as 100 ppm. The effect of organic insecticide endosulfan on alpha-amylase was also seen at very low concentrations (0.048 ppm). The electrophoretic analysis also revealed the inhibition of enzyme biosynthesis at the tested toxicant concentrations. (C) 2003 Elsevier Science Inc. All rights reserved.en_US
dc.identifier.doi10.1016/S0141-0229(03)00025-5
dc.identifier.endpage664en_US
dc.identifier.issn0141-0229
dc.identifier.issn1879-0909
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-0037457684
dc.identifier.scopusqualityQ1
dc.identifier.startpage658en_US
dc.identifier.urihttps://doi.org/10.1016/S0141-0229(03)00025-5
dc.identifier.urihttps://hdl.handle.net/11468/16252
dc.identifier.volume32en_US
dc.identifier.wosWOS:000182815300003
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofEnzyme and Microbial Technology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectBeta-Galactosidaseen_US
dc.subjectEnzyme Inhibitionen_US
dc.subjectHeavy Metalsen_US
dc.subjectOrganic Pesticides Permeabilityen_US
dc.titleA comparative study of bioassays based on enzyme biosynthesis in Escherichia coli and Bacillus subtilis exposed to heavy metals and organic pesticidesen_US
dc.titleA comparative study of bioassays based on enzyme biosynthesis in Escherichia coli and Bacillus subtilis exposed to heavy metals and organic pesticides
dc.typeArticleen_US

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