A comparative study of bioassays based on enzyme biosynthesis in Escherichia coli and Bacillus subtilis exposed to heavy metals and organic pesticides
[ X ]
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Two enzyme assays, namely beta-galactosidase in Escherichia coli (E.C.3.2.1.23) and alpha-amylase in Bacillus subtilis (E.C.3.2.1.1), were compared following exposure to various concentrations of heavy metals and organic pesticides. It was shown that alpha-amylase biosynthesis in the Gram-positive bacteria was more sensitive to heavy metals, particularly to mercury as low as 0.1 ppm, compared to beta-galactosidase in the Gram-negative bacteria. The results of organic pesticide treatment were more clear-cm when comparing both assays. Alpha-amylase test system can detect the inhibition by the organometallic fungicides Maneb and Mancozeb at as low as 0.1 ppm, while no inhibition with beta-galactosidase system up to as high as 100 ppm. The effect of organic insecticide endosulfan on alpha-amylase was also seen at very low concentrations (0.048 ppm). The electrophoretic analysis also revealed the inhibition of enzyme biosynthesis at the tested toxicant concentrations. (C) 2003 Elsevier Science Inc. All rights reserved.
Açıklama
Anahtar Kelimeler
Alpha-Amylase, Beta-Galactosidase, Enzyme Inhibition, Heavy Metals, Organic Pesticides Permeability
Kaynak
Enzyme and Microbial Technology
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
Cilt
32
Sayı
6
