Hepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study

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dc.contributor.authorErisgin, Zuleyha
dc.contributor.authorMutlu, Hasan Serdar
dc.contributor.authorTekelioglu, Yavuz
dc.contributor.authorDeveci, Engin
dc.contributor.authorSeker, Ugur
dc.date.accessioned2024-04-24T17:08:08Z
dc.date.available2024-04-24T17:08:08Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis study aims to investigate the effects of melamine exposure from the weaning period (21st postnatal days in rats) on liver tissue. Female Wistar albino rats (n = 18) were divided into three groups. About 0.1-ml saline was applied to the control group by gavage for 21 days from the postnatal 21st day. The second group was taken 50-mg/kg melamine (in 0.1-ml saline) and the third group was taken 75-mg/kg melamine (in 0.1-ml saline) p.o. On the postnatal 45th day, all rats were sacrificed under anesthesia. Then, liver tissues were cut into three parts and two of them placed in neutral formalin for histopathological and flow cytometric analysis, and one of them placed in 2.5% glutaraldehyde. Histopathological analysis was performed with hematoxylin & eosin, Masson trichrome, periodic acid Schiff stained sections, and also with transmission electron microscopy. Apoptosis (Annexin V positivity) was analyzed by flow cytometry. According to histopathological analysis, hepatocyte damage, sinusoidal dilatation, and inflammatory cell infiltration significantly increased in both melamine groups compared with the control group. Apoptosis significantly increased in the 50 and 75-mg melamine groups compared with the control group. In the results of transmission electron microscopy analysis, there was abnormal chromatin distribution in the hepatocyte nuclei, loss in the cristae of the mitochondria, and organelle loss in large areas in the cytoplasm in both melamine exposure groups. As result, melamine exposure from the weaning period causes liver damage with increasing doses. [GRAPHICS] .en_US
dc.identifier.doi10.1093/toxres/tfab022
dc.identifier.endpage424en_US
dc.identifier.issn2045-452X
dc.identifier.issn2045-4538
dc.identifier.issue3en_US
dc.identifier.pmid34141155
dc.identifier.scopus2-s2.0-85111826119
dc.identifier.scopusqualityQ3
dc.identifier.startpage418en_US
dc.identifier.urihttps://doi.org/10.1093/toxres/tfab022
dc.identifier.urihttps://hdl.handle.net/11468/17215
dc.identifier.volume10en_US
dc.identifier.wosWOS:000671151100006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherOxford Univ Pressen_US
dc.relation.ispartofToxicology Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectApoptosisen_US
dc.subjectElectron Microscopyen_US
dc.subjectFlow Cytometryen_US
dc.subjectHepatotoxicityen_US
dc.subjectMelamineen_US
dc.subjectWeaning Perioden_US
dc.subjectRaten_US
dc.titleHepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic studyen_US
dc.titleHepatotoxic effects of melamine exposure from the weaning period in rats: a flow cytometric, electron microscopic, and histopathologic study
dc.typeArticleen_US

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