Cloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerus

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dc.contributor.authorTunca, S
dc.contributor.authorYilmaz, EI
dc.contributor.authorPiret, J
dc.contributor.authorLiras, P
dc.contributor.authorÖzcengiz, G
dc.date.accessioned2024-04-24T16:18:19Z
dc.date.available2024-04-24T16:18:19Z
dc.date.issued2004
dc.departmentDicle Üniversitesien_US
dc.description.abstractCarbon flow through the lysine branch of the aspartate biosynthetic pathway is a rate-limiting step in the formation of cephamycin C, a broad spectrum P-lactam antibiotic produced by Streptomyces clavuligerus. In this study, genes which encode the enzymes catalyzing the first two steps of the aspartate pathway, ask (aspartokinase) and asd (aspartate semialdehyde dehydrogenase), in S. clavuligerus NRRL 3585 were cloned and sequenced. Nucleotide sequencing and codon preference analysis revealed three complete open reading frames (ORFs). ORF2 starts within ORFI and terminates by utilizing the same stop codon as ORFI, an arrangement typical of many ask genes. ORF3 is located 2 nucleotides downstream of ORF1,2. Database comparisons with these proteins identified ORFI as the large (a) subunit of aspartokinase, ORF2 as the small (P) subunit of aspartokinase and ORF3 as the aspartate semialdehyde dehydrogenase. The cloned genes were functionally expressed in auxotrophic Escherichia coli strains, CGSC5074 (ask(-)) and E. coli CGSC5080 (asd(-)), the two enzymes were partially purified from E. coli cell extracts and their kinetic parameters were determined. The effects of end product amino acids and diaminopimelic acid on the activity of Ask and Asd enzymes were also described. (C) 2004 Elsevier SAS. All rights reserved.en_US
dc.identifier.doi10.1016/j.resmic.2004.03.007
dc.identifier.endpage534en_US
dc.identifier.issn0923-2508
dc.identifier.issn1769-7123
dc.identifier.issue7en_US
dc.identifier.pmid15313252
dc.identifier.scopus2-s2.0-4143068328
dc.identifier.scopusqualityQ3
dc.identifier.startpage525en_US
dc.identifier.urihttps://doi.org/10.1016/j.resmic.2004.03.007
dc.identifier.urihttps://hdl.handle.net/11468/15992
dc.identifier.volume155en_US
dc.identifier.wosWOS:000223769300005
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofResearch in Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAsk-Asden_US
dc.subjectCephamycin Cen_US
dc.subjectAspartate Pathwayen_US
dc.subjectBeta-Lactam Antibioticsen_US
dc.subjectAspartokinaseen_US
dc.subjectAspartate Semialdehyde Dehydrogenaseen_US
dc.subjectPurificationen_US
dc.subjectRegulationen_US
dc.titleCloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerusen_US
dc.titleCloning, characterization and heterologous expression of the aspartokinase and aspartate semialdehyde dehydrogenase genes of cephamycin C-producer Streptomyces clavuligerus
dc.typeArticleen_US

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