Bioapplications of polythiophene-g-polyphenylalanine-covered surfaces
Yükleniyor...
Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Wiley-V C H Verlag Gmbh
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The fabrication of electro and bioactive surfaces by electrochemical deposition of the thiophene-functionalized polyphenylalanine macromonomer (T-g-PPhe) is reported. The resulting conducting graft copolymer, polythiophene-graft-polyphenylalanine (PT-g-PPhe) formed on the indium tin oxide (ITO) glass surface, is characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, and fluorescence microscopy. Then, possible uses of PT-g-PPhe as matrices in the sensor design for both electrochemical biosensing and cell adhesion studies are investigated. In the first part, PT-g-PPhe that is deposited on ITO is further functionalized with the arginylglycylaspartic acid peptide via 1-Ethyl-3-(3 dimethylaminopropyl) carbodiimide for the selective cell adhesion. Immunofluorescence staining is performed to detect the difference between adherences of integrin alpha v beta 3 receptor positive (U87-MG) and negative (HaCaT) cell lines on to the biofunctional surface. In the second part, an electrochemical glucose sensor is constructed by immobilizing glucose oxidase on the surface of PT-g-PPhe, which is deposited on a glassy carbon electrode.
Açıklama
Anahtar Kelimeler
Cell adhesion, Electropolymerization, Graft copolymer, Polypeptide macromonomer, Surface design
Kaynak
Macromolecular Chemistry and Physics
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
216
Sayı
18
Künye
Güler, E., Akbulut, H., Bozokalfa, G., Demir, B., Eyrilmez, G. O., Yavuz, M. ve diğerleri. (2015). Bioapplications of polythiophene-g-polyphenylalanine-covered surfaces. Macromolecular Chemistry and Physics, 216(18), 1868-1878.
