Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy

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dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorKurar, Ercan
dc.contributor.authorKayis, Seyit A.
dc.contributor.authorAslan, Selim
dc.contributor.authorSemacan, Ahmet
dc.contributor.authorCelik, Sefa
dc.contributor.authorGuzeloglu, Aydin
dc.date.accessioned2024-04-24T16:10:48Z
dc.date.available2024-04-24T16:10:48Z
dc.date.issued2010
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim was to evaluate expression of genes involved in the biosynthesis of prostaglandins (PTG), Prostaglandin H Synthase-1 (PTGS1) and PTGS2, PGF synthase (PTGFS), and PGE synthase (PTGES), PGF receptor (PTGFR). PGE receptors (PTGER2 and FTGER4), prostaglandin transporter (SLCO2A1) and hydroxyprostaglandin dehydrogenase-15 (HPGD). Endometrial biopsies were obtained from mares on day of ovulation (d0, n = 4), late diestrus (LD, n = 4), early luteolysis (EL n = 4) and after luteolysis (AL n = 4) during the cycle. Stages of the cycle were confirmed by plasma progesterone concentrations measured daily and ultrasound examinations. Biopsies were also taken on days 14 (P14; n = 4), 15 (P15. n = 4), 18 (P18, n = 4) and 22 (P22; n = 4) of pregnancy. Relative mRNA expressions were quantified using real-time RT-PCR. A mixed model was fitted on the normalized data and least significant difference test (alpha = 0.05) was employed. Expression of PTGS1 mRNA was low throughout the estrous cycle and early days of pregnancy, but upregulated on P18 and P22. PTGS2 expression was increased on EL but it was suppressed by pregnancy on P15, P18, and P22. PTGFS expression was upregulated in both cyclic and pregnant mares compared to d0 and its level was the highest on LD. PTGFR expression was transiently increased on LD and EL and was suppressed during early pregnancy. Both PTGES and PTGER2 expressions were increased on LD, El, and early pregnancy, but were decreased after the luteolysis in cyclic mares as they remained high on P18 and P22. PTGER4 expression did not change throughout the cycle and early pregnancy. Levels of HPGD and SLCO2A1 were significantly increased only on P22. In conclusion, PTGS2 expression increases around the time of luteolysis and concurrent upregulation of PTGFS and PTGES indicates that equine endometrium has increased capability of PTG production around the time of luteolysis. However, pregnancy reduces PTGS2 expression, but maintains the high levels of PTGES during early pregnancy along with PTGER2 while PTGFR expression was suppressed. These findings suggest that possible luteotrophic action of PGE(2) is required in early equine pregnancy. PTGS1 is only upregulated later in the early pregnancy suggesting that it is not involved in luteolysis, but could be the main PTGS enzyme at this time during early pregnancy. An increase in HPGD and SLCO2A1 levels on P22 indicates a tight regulation of PTG action by pregnancy. (C) 2010 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TOVAG 1070035, TOVAG 1050652]en_US
dc.description.sponsorshipThis study was financially supported by The Scientific and Technological Research Council of Turkey (TUBITAK) grant TOVAG 1070035 (to AG). MOA was financially supported by TOVAG 1050652. The authors would like to thank the staff at the equestrian center of Selcuk University for handling and care of the mares used in this study. The animal material was kindly provided by TIGEM Anatolian Agricultural Station Horse Breeding Center, Eskisehir, Turkey and TIGEM Karacabey Horse Breeding Center, Bursa, Turkey.en_US
dc.identifier.doi10.1016/j.anireprosci.2010.08.007
dc.identifier.endpage132en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.issue1-2en_US
dc.identifier.pmid20832957
dc.identifier.scopus2-s2.0-77957676691
dc.identifier.scopusqualityQ1
dc.identifier.startpage124en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2010.08.007
dc.identifier.urihttps://hdl.handle.net/11468/15118
dc.identifier.volume122en_US
dc.identifier.wosWOS:000283913600015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMareen_US
dc.subjectPregnancyen_US
dc.subjectEndometriumen_US
dc.subjectProstaglandin Systemen_US
dc.titleEvaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancyen_US
dc.titleEvaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy
dc.typeArticleen_US

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