RP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species

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dc.contributor.authorTohma, Hatice
dc.contributor.authorKoksal, Ekrem
dc.contributor.authorKilic, Omer
dc.contributor.authorAlan, Yusuf
dc.contributor.authorYilmaz, Mustafa Abdullah
dc.contributor.authorGulcin, Ilhami
dc.contributor.authorBursal, Ercan
dc.date.accessioned2024-04-24T17:20:50Z
dc.date.available2024-04-24T17:20:50Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe identification and quantification of the phenolic contents of methanolic extracts of three Salvia L. species namely S. brachyantha (Bordz.) Pobed, S. aethiopis L., and S. microstegia Boiss. and Bal. were evaluated using reverse phase high performance liquid chromatography, UV adsorption, and mass spectrometry (RP-HPLC/MS). In order to determine the antioxidant capacity of these species, cupric ions (Cu2+) reducing assay (CUPRAC) and ferric ions (Fe3+) reducing assay (FRAP) were performed to screen the reducing capacity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was employed for evaluation of the radical scavenging activity for both solvents. In further investigation, the antimicrobial activities of Salvia species were tested using the disc diffusion method against three Gram-positive and four Gram-negative microbial species, as well as three fungi species. The results showed that there is a total of 18 detectable phenols, the most abundant of which was kaempferol in S. microstegia and rosmarinic acids in S. brachyantha and S aethiopis. The other major phenols were found to be apigenin, luteolin, p-coumaric acid, and chlorogenic acid. All species tested showed moderate and lower antioxidant activity than standard antioxidants such as butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and ascorbic acid. The ethanolic extracts of Salvia species revealed a wide range of antimicrobial activity. S. brachyantha and S. microstegia showed the highest antimicrobial activities against B. subtilis, whereas S. aethiopis was more effective on Y. lipolytica. None of the extracts showed anti-fungal activity against S. cerevisiae. Thus these species could be valuable due to their bioactive compounds.en_US
dc.description.sponsorshipResearch Chairs Program at King Saud Universityen_US
dc.description.sponsorshipIlhami Gulcin and Saleh H. Alwasel would like to extend their sincere appreciation to the Research Chairs Program at King Saud University for providing funding for this research.en_US
dc.identifier.doi10.3390/antiox5040038
dc.identifier.issn2076-3921
dc.identifier.issue4en_US
dc.identifier.pmid27775656
dc.identifier.scopus2-s2.0-85010303899
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.3390/antiox5040038
dc.identifier.urihttps://hdl.handle.net/11468/19266
dc.identifier.volume5en_US
dc.identifier.wosWOS:000392281400006
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofAntioxidants
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectSalvia L. Speciesen_US
dc.subjectAntioxidant Activityen_US
dc.subjectAntimicrobial Activityen_US
dc.subjectHplc-Ms/Msen_US
dc.subjectPhenolic Compoundsen_US
dc.titleRP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Speciesen_US
dc.titleRP-HPLC/MS/MS Analysis of the Phenolic Compounds, Antioxidant and Antimicrobial Activities of Salvia L. Species
dc.typeArticleen_US

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