Enzyme inhibitory function and phytochemical profile of Inula discoidea using in vitro and in silico methods
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Many plant species have a large diversity of secondary metabolites with different biological activities. This study aims to assess the phenolic constituent, enzyme inhibitory and antioxidant activities of the aqueous (water) and methanol extracts of Inula discoidea. The enzyme assays showed effective enzyme inhibition of the methanol extract against acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glutathione S-transferase (GST), and alpha-glycosidase (alpha-Gly) enzymes. The IC50 values for AChE, BChE, GST, and alpha-Gly were found as 38.5 mg/mL, 34.65 mg/mL, 77.0 mg/mL, and 40.76 mg/mL, respectively. Antioxidant properties of the aqueous and methanol extracts of I. discoidea were determined by four well-known in vitro techniques (ABTS, CUPRAC, DPPH, and FRAP methods). The antioxidant values of both water and methanol extracts were found to be better than the standard antioxidants (BHA, BHT, ascorbic acid, and alpha-tocopherol) in ABTS and CUPRAC methods. According to an updated LC-MS/MS technique analysis, quinic acid (21.08 mg/g), protocatechuic acid (4.49 mg/g), and gallic acid (0.48 mg/g) were found as major phenolic compounds of the plant extract. The binding interactions of major phenolic compounds of I. discoidea with the AChE, BChE, GST, and alpha-Gly enzymes were investigated by the molecular docking studies.
Açıklama
Anahtar Kelimeler
Antioxidant, Enzyme inhibition, Inula discoidea, Molecular docking, LC-MS, MS
Kaynak
Biophysical Chemistry
WoS Q Değeri
Q2
Scopus Q Değeri
Q2
Cilt
277
Sayı
Künye
Bursal, E., Yılmaz, M.A., İzol, E., Türkan, F., Atalar, M.N., Murahari, M. ve diğerleri. (2021). Enzyme inhibitory function and phytochemical profile of Inula discoidea using in vitro and in silico methods. Biophysical Chemistry, 277, 1-7.
