Selective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprinting

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dc.authorid0000-0002-2095-7614en_US
dc.contributor.authorDolak, İbrahim
dc.contributor.authorKeçili, Rüstem
dc.contributor.authorYılmaz, Filiz
dc.contributor.authorErsöz, Arzu
dc.contributor.authorSay, Rıdvan
dc.date.accessioned2022-06-03T11:14:25Z
dc.date.available2022-06-03T11:14:25Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Diyarbakır Teknik Bilimler Meslek Yüksekokulu, Kimya ve Kimyasal İşleme Teknolojileri Bölümüen_US
dc.descriptionWOS:000620937600003
dc.description.abstractRecognition and determination of ubiquitin (Ubq) is very important since it affects the enzymatic mechanisms, which causes damages in the living organisms. This can lead to diseases especially in neuronal system. Molecularly imprinted polymers (MIPs) are selective resins that exhibit high affinity toward target compounds. MIPs have attracted significicant research interest because of their various applications in solid phase extraction, biosensors and drug delivery, etc. Various MIP types can be used such as thermo-sensitive, pH-sensitive, biomolecule-sensitive, ion-sensitive and photo-sensitive in these applications. Photosensitive MIPs can be used to increase the selectivity and binding efficiency to decrease denaturation of target biomolecules. The aim of this study is to prepare photosensitive cross-linked p(Ubq-co-MACys-Ru(bipyr)(2)-MACys) polymeric nanoparticles (UbqINPs) and leach with 0.5 M HCl to create Ubq memories onto nanoparticles (UbqINPs) and optimize their binding parameters in cryogel based column system. For this purpose, different factors such as pH, flow rate, ionic strength and temperature were investigated, and maximum ubiquitin binding capacity was found to be 25 mg/g at pH 7. The results showed that the UbqINPs can be considered as a good binding material for recognition and separation of Ubq.en_US
dc.identifier.citationDolak, İ., Keçili, R., Yılmaz, F., Ersöz, A. ve Say, R (2021). Selective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprinting. Journal of Analytical Chemistry, 76(2), 165-171.en_US
dc.identifier.doi10.1134/S1061934821020040
dc.identifier.endpage171en_US
dc.identifier.issn1061-9348
dc.identifier.issn1608-3199
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-85101580830
dc.identifier.scopusqualityQ3
dc.identifier.startpage165en_US
dc.identifier.urihttps://link.springer.com/content/pdf/10.1134/S1061934821020040.pdf
dc.identifier.urihttps://hdl.handle.net/11468/9960
dc.identifier.volume76en_US
dc.identifier.wosWOS:000620937600003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorDolak, İbrahim
dc.language.isoenen_US
dc.publisherPleiades Publishing INC.en_US
dc.relation.ispartofJournal of Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPhotosensitive covalently imprinted polymersen_US
dc.subjectUbiquitinen_US
dc.subjectNanoparticlesen_US
dc.subjectRecognitionen_US
dc.subjectSeparationen_US
dc.titleSelective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprintingen_US
dc.titleSelective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprinting
dc.typeArticleen_US

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