Selective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprinting
Yükleniyor...
Tarih
2021
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Pleiades Publishing INC.
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Recognition and determination of ubiquitin (Ubq) is very important since it affects the enzymatic mechanisms, which causes damages in the living organisms. This can lead to diseases especially in neuronal system. Molecularly imprinted polymers (MIPs) are selective resins that exhibit high affinity toward target compounds. MIPs have attracted significicant research interest because of their various applications in solid phase extraction, biosensors and drug delivery, etc. Various MIP types can be used such as thermo-sensitive, pH-sensitive, biomolecule-sensitive, ion-sensitive and photo-sensitive in these applications. Photosensitive MIPs can be used to increase the selectivity and binding efficiency to decrease denaturation of target biomolecules. The aim of this study is to prepare photosensitive cross-linked p(Ubq-co-MACys-Ru(bipyr)(2)-MACys) polymeric nanoparticles (UbqINPs) and leach with 0.5 M HCl to create Ubq memories onto nanoparticles (UbqINPs) and optimize their binding parameters in cryogel based column system. For this purpose, different factors such as pH, flow rate, ionic strength and temperature were investigated, and maximum ubiquitin binding capacity was found to be 25 mg/g at pH 7. The results showed that the UbqINPs can be considered as a good binding material for recognition and separation of Ubq.
Açıklama
WOS:000620937600003
Anahtar Kelimeler
Photosensitive covalently imprinted polymers, Ubiquitin, Nanoparticles, Recognition, Separation
Kaynak
Journal of Analytical Chemistry
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
76
Sayı
2
Künye
Dolak, İ., Keçili, R., Yılmaz, F., Ersöz, A. ve Say, R (2021). Selective recognition and separation of ubiquitin by nanoparticle embedded cryogel traps with ubiquitin memories based on photosensitive covalent imprinting. Journal of Analytical Chemistry, 76(2), 165-171.
