IMPACTS OF SPECIFIC CRYOPROTECTANTS ON SPERM FREEZING AND RELATIONSHIPS BETWEEN CRYODAMAGE AND OXIDATION STRESS PARAMETERS IN AWASSI RAM SPERM

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dc.contributor.authorVarisli, Omer
dc.contributor.authorErat, Serkan
dc.contributor.authorBozkaya, Faruk
dc.contributor.authorAydilek, Nurettin
dc.contributor.authorTaskin, Abdullah
dc.date.accessioned2024-04-24T17:37:31Z
dc.date.available2024-04-24T17:37:31Z
dc.date.issued2021
dc.departmentDicle Üniversitesien_US
dc.description.abstractBACKGROUND: The role of oxidative stress during cryoprotectant treatment has received little attention. OBJECTIVE: To assess the effects of different cryoprotectants and discover relationships between cryodamage and oxidative stress parameters on Awassi ram sperm. MATERIALS AND METHODS: The sperm samples diluted with Salamon's tris-citrate (TRIS) containing 20% centrifuged egg yolk and 0.5, 1.0 or 1.5 M Glycerol (Gly), methanol (M), 2-methoxyethanol (2-ME), dimethylacetamide (DMA) and 1.2 propanediol (PR). After 2 h of equilibration at +4 degrees C, the sperm samples were frozen in liquid nitrogen vapour and stored. RESULTS: The best post-thaw motility (43.3%, 41.7%) of sperm was achieved when protected with 0.5 and 1.0 M glycerol. Arylesterase and ceruloplasmin parameters were significantly different after equilibration, whereas sulfhydryl groups were significantly different after freezing in their respective groups (P< 0.05). CONCLUSION: The increased use of glycerol caused greater loss of motility. The role of oxidative stress in freezing was also found to be limited.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [112O911]en_US
dc.description.sponsorshipThis work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) grant number 112O911. I would like to thank my fellow graduate students for their help.en_US
dc.identifier.endpage95en_US
dc.identifier.issn0143-2044
dc.identifier.issn1742-0644
dc.identifier.issue2en_US
dc.identifier.pmid33970985
dc.identifier.scopus2-s2.0-85105723114
dc.identifier.scopusqualityQ3
dc.identifier.startpage87en_US
dc.identifier.urihttps://hdl.handle.net/11468/20991
dc.identifier.volume42en_US
dc.identifier.wosWOS:000636820500005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherCryo Lettersen_US
dc.relation.ispartofCryoletters
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAwassien_US
dc.subjectCryopreservationen_US
dc.subjectCryoprotectantsen_US
dc.subjectOxidative Stressen_US
dc.subjectRamen_US
dc.subjectSpermen_US
dc.titleIMPACTS OF SPECIFIC CRYOPROTECTANTS ON SPERM FREEZING AND RELATIONSHIPS BETWEEN CRYODAMAGE AND OXIDATION STRESS PARAMETERS IN AWASSI RAM SPERMen_US
dc.titleIMPACTS OF SPECIFIC CRYOPROTECTANTS ON SPERM FREEZING AND RELATIONSHIPS BETWEEN CRYODAMAGE AND OXIDATION STRESS PARAMETERS IN AWASSI RAM SPERM
dc.typeArticleen_US

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