The Optimization of a Rapid Pulsed-Field Gel Electrophoresis Protocol for the Typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp.

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dc.contributor.authorDurmaz, Riza
dc.contributor.authorOtlu, Baris
dc.contributor.authorKoksal, Fatih
dc.contributor.authorHosoglu, Salih
dc.contributor.authorOzturk, Recep
dc.contributor.authorErsoy, Yasemin
dc.contributor.authorAktas, Elif
dc.date.accessioned2024-04-24T17:39:57Z
dc.date.available2024-04-24T17:39:57Z
dc.date.issued2009
dc.departmentDicle Üniversitesien_US
dc.description.abstractPulsed-field gel electrophoresis (PFGE) is the most common genotyping method used for the typing of a number of bacterial species. Generally, investigators use their own custom-developed protocol, but a standardized PFGE protocol would allow the comparison of typing results between laboratories and the tracing of strains around the country. In the present study, we optimized a PFGE protocol for subtyping of Acinetobacter baumannii, Escherichia coli and Klebsiella spp., which are commonly isolated from nosocomial infections in many hospitals. Reproducibility of our PFGE procedure was studied three times at 2- to 3-week intervals. Epidemiological concordance of the optimized PFGE procedure was tested on seven isolates of A. baumannii from a previous outbreak and seven A. baumannii isolates randomly selected among the clinical isolates. The optimized PFGE procedure was evaluated on a total of 174 clinical isolates including 62 A. baumannii, 50 E. coli and 62 Klebsiella spp. The inter-laboratory reproducibility of the optimized protocol was tested at four laboratories. The optimized procedure is completed in 28 h after culturing. It is likely to be cost-effective, due to the reduction in the time, reagent volume and enzyme concentration needed. The procedure showed high concordance with epidemiological data. There were no non-typeable isolates among the tested bacteria. It is reproducible and versatile. This protocol can be used to identify outbreaks and monitor the spreading rate of nosocomial infections caused by the tested bacterial isolates. Furthermore, due to its high intra- and inter-laboratory reproducibility, the protocol has the potential to be useful for comparing PFGE fingerprinting profiles of the isolates from different settings.en_US
dc.description.sponsorshipTUBITAK Ankara/Turkey [106S211 (SBAG-3459)]en_US
dc.description.sponsorshipThis research was funded by TUBITAK Ankara/Turkey, with project number 106S211 (SBAG-3459).en_US
dc.identifier.endpage377en_US
dc.identifier.issn1344-6304
dc.identifier.issn1884-2836
dc.identifier.issue5en_US
dc.identifier.pmid19762987
dc.identifier.scopus2-s2.0-72849129076
dc.identifier.scopusqualityQ2
dc.identifier.startpage372en_US
dc.identifier.urihttps://hdl.handle.net/11468/21457
dc.identifier.volume62en_US
dc.identifier.wosWOS:000270557900008
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherNatl Inst Infectious Diseasesen_US
dc.relation.ispartofJapanese Journal of Infectious Diseases
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[No Keyword]en_US
dc.titleThe Optimization of a Rapid Pulsed-Field Gel Electrophoresis Protocol for the Typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp.en_US
dc.titleThe Optimization of a Rapid Pulsed-Field Gel Electrophoresis Protocol for the Typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp.
dc.typeArticleen_US

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