Immunolocalization of vascular endothelial growth factor, its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bitch uterus during the sexual cycle

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dc.contributor.authorSagsoz, Hakan
dc.contributor.authorLiman, Narin
dc.contributor.authorKucukaslan, Ibrahim
dc.contributor.authorSaruhan, Berna Guney
dc.date.accessioned2024-04-24T16:10:48Z
dc.date.available2024-04-24T16:10:48Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractAngiogenesis is regulated by proangiogenic and antiangiogenic factors. Vascular endothelial growth factor (VEGF) is a prime proangiogenic regulator, whereas vascular endothelial growth inhibitor (VEGI) is a specific antiangiogenic cytokine. To clarify temporal changes in the localization of pro-angiogenic and anti-angiogenic factors in the uterus of normal bitches during the proestrus, estrus, diestrus and anestrus phases of the estrous cycle, the expressions of VEGF and its receptors (flt1/fms, flk1/KDR and flt4) and their correlation with VEGI were analyzed using immunohistochemistry. Uteruses were collected after ovariohysterectomy. Immunohistochemical staining was evaluated semi-quantitatively by an immunohistochemical total score consisting of the sum of the intensity and proportional scores. The results in the bitch uterus demonstrated that positive immunohistochemical staining was found exclusively in the cytoplasm and apical membrane of luminal and glandular epithelial, stromal and smooth muscle cells and nuclear staining was observed in the fit1/fms, flk4 and VEGI during proestrous and estrous. Semi-quantitative analyses revealed that the total score for VEGF in the glandular epithelial cells was significantly higher than that of luminal, endometrial stromal and myometrial smooth muscle cells during proestrous (p < 0.05). The total score for flk1/KDR and flt4 in the glandular epithelium was also significantly higher than that of endometrial stromal cells during proestrous, whilst the total score for flt1/fms in the glandular epithelium was significantly higher than that of endometrial stromal cells during anestrus (p < 0.05). We conclude that, in the bitch uterus, cyclic changes may be precisely regulated by the combined functions of VEGF family members, angiogenic VEGF and VEGF receptors, and the angiogenesis inhibitor VEGI. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.identifier.doi10.1016/j.anireprosci.2013.05.014
dc.identifier.endpage254en_US
dc.identifier.issn0378-4320
dc.identifier.issn1873-2232
dc.identifier.issue3-4en_US
dc.identifier.pmid23800852
dc.identifier.scopus2-s2.0-84881375620
dc.identifier.scopusqualityQ1
dc.identifier.startpage241en_US
dc.identifier.urihttps://doi.org/10.1016/j.anireprosci.2013.05.014
dc.identifier.urihttps://hdl.handle.net/11468/15122
dc.identifier.volume140en_US
dc.identifier.wosWOS:000324151700017
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofAnimal Reproduction Science
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBitch Uterusen_US
dc.subjectVegf Receptorsen_US
dc.subjectVegien_US
dc.subjectEstrous Cycleen_US
dc.titleImmunolocalization of vascular endothelial growth factor, its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bitch uterus during the sexual cycleen_US
dc.titleImmunolocalization of vascular endothelial growth factor, its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bitch uterus during the sexual cycle
dc.typeArticleen_US

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