Purification of glucose 6-phosphate dehydrogenase from Buffalo (Bubalus bubalis) erythrocytes and investigation of some kinetic properties

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dc.contributor.authorÇiftçi, M
dc.contributor.authorBeydemir, S
dc.contributor.authorYilmaz, H
dc.contributor.authorAltikat, S
dc.date.accessioned2024-04-24T16:18:57Z
dc.date.available2024-04-24T16:18:57Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractGlucose 6-phosphate dehydrogenase (G6PD) was purified from buffalo (Bubalus bubalis) erythrocytes and some characteristics of the enzyme were investigated. The purification procedure was composed of two steps: hemolysate preparation and 2',5'-ADP Sepharose 4B affinity gel chromatography. Thanks to the two consecutive procedures, the enzyme, having a specific activity of 69.7 EU/mg proteins, was purified 650-fold with a yield of 31%. Optimal pH, stable pH, optimal temperature, molecular weight, and K-m and V-max values for NADP(+) and glucose 6-phosphate (G6-P) substrates were also determined for the enzyme. In addition K-i values and the type of inhibition were determined by means of Lineweaver-Burk graphs obtained for such inhibitors as ATP, ADP, NADPH, and NADH. (C) 2003 Elsevier Science (USA). All rights reserved.en_US
dc.identifier.doi10.1016/S1046-5928(03)00073-1
dc.identifier.endpage310en_US
dc.identifier.issn1046-5928
dc.identifier.issn1096-0279
dc.identifier.issue2en_US
dc.identifier.pmid12767823
dc.identifier.scopus2-s2.0-0038467604
dc.identifier.scopusqualityQ3
dc.identifier.startpage304en_US
dc.identifier.urihttps://doi.org/10.1016/S1046-5928(03)00073-1
dc.identifier.urihttps://hdl.handle.net/11468/16347
dc.identifier.volume29en_US
dc.identifier.wosWOS:000183382100022
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherAcademic Press Inc Elsevier Scienceen_US
dc.relation.ispartofProtein Expression and Purification
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBuffaloen_US
dc.subjectPurificationen_US
dc.subjectGlucose 6-Phosphate Dehydrogenaseen_US
dc.subjectErythrocyteen_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from Buffalo (Bubalus bubalis) erythrocytes and investigation of some kinetic propertiesen_US
dc.titlePurification of glucose 6-phosphate dehydrogenase from Buffalo (Bubalus bubalis) erythrocytes and investigation of some kinetic properties
dc.typeArticleen_US

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