Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract

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dc.contributor.authorKandemir, Sevgi İrtegun
dc.contributor.authorAktepe, Necmettin
dc.contributor.authorBaran, Ayse
dc.contributor.authorBaran, Mehmet Fırat
dc.contributor.authorAtalar, Mehmet Nuri
dc.contributor.authorKeskin, Cumali
dc.contributor.authorKaradağ, Musa
dc.date.accessioned2024-04-24T16:02:32Z
dc.date.available2024-04-24T16:02:32Z
dc.date.issued2024
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Biyoloji Anabilim Dalıen_US
dc.description.abstractPurpose The phenolic composition, antioxidant, antimicrobial activity, enzyme inhibition activity, and cytotoxic activity potentials of the plant Rumex acetosella L. (R. acetosella) were examined in this study. Materials and Methods: The chemical composition of R. acetosella methanol extract was identified by the LC-MS/MS method. The antioxidant activity was tested using beta-carotene/linoleic acid, DPPH free radical scavenging, ABTS cation radical scavenging, CUPRAC reducing power, and metal chelating activity methods. The cytotoxic activity was determined by the MTT assay using human ovarian adenocarcinoma (Skov-3), glioblastoma (U87), human dermal fibroblasts (HDF), and human colorectal adenocarcinoma (CaCo-2) cell lines. The antimicrobial activity of methanolic extracts was tested on gram-negative (Escherichia coli and Pseudomonas aeuriginosa) and gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) using the in vitro minimum inhibition concentration method (MIC). Enzyme inhibition activity of R. acetosella methanol extract was measured spectrophotometrically against acetylcholinesterase (AChE) and glutathione S-transferase (GST) enzymes. Results: The findings showed that the major components of the methanol extract content were luteolin-7-O-glucoside (1.599 m/L), polydatin (91,024 m/L), and shikimic acid (0.773 m/L). It was determined that the extract and standard antioxidant (a-tocopherol) results in DPPH center dot, and ABTS center dot + tests performed to determine the antioxidant activity were close to each other, and this value was more effective than the standard antioxidant (alpha-tocopherol) in the CUPRAC test. These results suggested that the plant's antioxidant potential was higher when compared with reference antioxidant compounds. It was determined that the methanol extract of R. acetosella had a weaker effect on the growth of the tested microorganisms than the antibiotics used as standard. The activity of the GST and AChE enzymes was found to be severely inhibited by the methanol extract of R. acetosella. Conclusion: Based on these findings, R. acetosella L. is a medicinal and commercially beneficial plant that warrants further investigation.en_US
dc.description.sponsorshipResearch Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences [73733]; Mardin Artuklu University; [MAU.BAP.23.SBF.016]en_US
dc.description.sponsorshipThis work was supported by Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences (Grant no: 73733) and also by Mardin Artuklu University (Project No. MAU.BAP.23.SBF.016).en_US
dc.identifier.citationKandemir, S. İ., Aktepe, N., Baran, A., Baran, M. F., Atalar, M. N., Keskin, C. ve diğerleri. (2024). Determination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract. Chemical Papers, 1-10.
dc.identifier.doi10.1007/s11696-024-03419-0
dc.identifier.issn0366-6352
dc.identifier.issn2585-7290
dc.identifier.scopus2-s2.0-85189916791
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11696-024-03419-0
dc.identifier.urihttps://hdl.handle.net/11468/14826
dc.identifier.urihttps://link.springer.com/article/10.1007/s11696-024-03419-0
dc.identifier.wosWOS:001199261100003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorKandemir, Sevgi İrtegün
dc.language.isoenen_US
dc.publisherSpringer Int Publ Agen_US
dc.relation.ispartofChemical Papers
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChemical compositionen_US
dc.subjectAntioxidanten_US
dc.subjectAntimicrobialen_US
dc.subjectEnzyme inhibitionen_US
dc.subjectCytotoxicityen_US
dc.subjectRumex acetosella L.en_US
dc.titleDetermination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extracten_US
dc.titleDetermination of chemical composition and antioxidant, cytotoxic, antimicrobial, and enzyme inhibition activities of Rumex acetosella L. plant extract
dc.typeArticleen_US

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