Influence of L-NAME and L-Arg on ischaemia-reperfusion induced gastric mucosa damage

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dc.contributor.authorÖztürk, H
dc.contributor.authorKara, IH
dc.contributor.authorOtçu, S
dc.contributor.authorKilinc, N
dc.contributor.authorYagmur, Y
dc.date.accessioned2024-04-24T17:37:32Z
dc.date.available2024-04-24T17:37:32Z
dc.date.issued2002
dc.departmentDicle Üniversitesien_US
dc.description.abstractObjective : The aim of this study was to investigate effects of L-NAME and L-Arginine on gastric mucosal injury induced by ischaemia-reperfusion. Methods : In the experiment, 20 New Zealand rabbits were used (2700-3000 g). Celiac artery was clamped for 30 min for ischaemia and then 60 min of reperfusion followed this after all rabbits were anaesthetized. In the Sham-control group (G 1, n = 5), laparotomy was performed, and the celiac artery was prepared without clipping. Group 2 (Untreated, n = 5) rabbits were only subjected to ischaemia-reperfusion. Group 3 (n = 5) rabbits had L-Arginine Methyl Ester (L-Arg) 3 mg/kg/min as IV infusion during the first 15 min of the reperfusion. Group 4 (n = 5) rabbits had a nitric oxide inhibitor NG-nitro-L-arginine methyl ester (L-NAME) 100 mug/kg/min IV during the first 15 min of the reperfusion. After 60 min of reperfusion, the rabbits were killed, and their stomachs were removed for histopathologic evaluation and determination of malondialdehyde (MDA) level. Results : After ischaemia-reperfusion, Untreated group had macroscopic necrosis involving 50 +/- 6% of total gastric mucosa area and deep mucosal necrosis involving 10 +/- 5% of mucosal strips. In the group treated with L-NAME, macroscopic mucosal necrosis involved 52 +/- 6% of total gastric mucosa area and deep mucosal necrosis involved 11 +/- 3 % of mucosal strips (both p > 0.05 versus Untreated group). L-Arg treatment significantly reduced macroscopic mucosal necrosis area to 20 +/- 6% and deep mucosal necrosis to 3 +/- 1% (both p < 0.05 versus Untreated group and L-NAME group). MDA level in the L-Arg group was significantly lower when compared to control and L-NAME group MDA level (p < 0.05). Conclusion : These results suggest that NO increase induced by L-Arginine injection is involved in the protection of gastric mucosa after isehaemia-reperfusion.en_US
dc.identifier.endpage+en_US
dc.identifier.issn0001-5644
dc.identifier.issue3en_US
dc.identifier.pmid12420606
dc.identifier.scopus2-s2.0-0036659480
dc.identifier.scopusqualityQ3
dc.identifier.startpage150en_US
dc.identifier.urihttps://hdl.handle.net/11468/21008
dc.identifier.volume65en_US
dc.identifier.wosWOS:000178750200003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherActa Medical Belgicaen_US
dc.relation.ispartofActa Gastro-Enterologica Belgica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGastric Mucosal Injuryen_US
dc.subjectIschaemia-Reperfusionen_US
dc.subjectNitric Oxideen_US
dc.subjectL-Arginine Methyl Esteren_US
dc.subjectNg-Nitro-L-Arginine Methyl Esteren_US
dc.titleInfluence of L-NAME and L-Arg on ischaemia-reperfusion induced gastric mucosa damageen_US
dc.titleInfluence of L-NAME and L-Arg on ischaemia-reperfusion induced gastric mucosa damage
dc.typeArticleen_US

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