Dynamics of microRNAs in bull spermatozoa

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dc.contributor.authorGovindaraju, Aruna
dc.contributor.authorUzun, Alper
dc.contributor.authorRobertson, LaShonda
dc.contributor.authorAtli, Mehmet O.
dc.contributor.authorKaya, Abdullah
dc.contributor.authorTopper, Einko
dc.contributor.authorCrate, Elizabeth A.
dc.date.accessioned2024-04-24T17:14:52Z
dc.date.available2024-04-24T17:14:52Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: MicroRNAs are small non-coding RNAs that regulate gene expression and thus play important roles in mammalian development. However, the comprehensive lists of microRNAs, as well as, molecular mechanisms by which microRNAs regulate gene expression during gamete and embryo development are poorly defined. The objectives of this study were to determine microRNAs in bull sperm and predict their functions. Methods: To accomplish our objectives we isolated miRNAs from sperm of high and low fertility bulls, conducted microRNA microarray experiments and validated expression of a panel of microRNAs using real time RT-PCR. Bioinformatic approaches were carried out to identify regulated targets. Results: We demonstrated that an abundance of microRNAs were present in bovine spermatozoa, however, only seven were differentially expressed; hsa-aga-3155, -8197, -6727, -11796, -14189, -6125, -13659. The abundance of miRNAs in the spermatozoa and the differential expression in sperm from high vs. low fertility bulls suggests that the miRNAs possibly play important functions in the regulating mechanisms of bovine spermatozoa. Conclusion: Identification of specific microRNAs expressed in spermatozoa of bulls with different fertility phenotypes will help better understand mammalian gametogenesis and early development.en_US
dc.description.sponsorshipMississippi Agriculture, Forestry, and Experiment Station; Alta Genetics, Inc.; National Science Foundation [DBI-1004842, EPS-0903787]; Mississippi INBRE; National Center for Research Resources [5P20RR016476-11]; National Institute of General Medical Sciences from the National Institutes of Health [8 P20 GM103476-11]; Div Of Biological Infrastructure; Direct For Biological Sciences [1004842] Funding Source: National Science Foundation; EPSCoR; Office Of The Director [1006983, 0903787] Funding Source: National Science Foundationen_US
dc.description.sponsorshipThis study was funded in part by Mississippi Agriculture, Forestry, and Experiment Station, and by Alta Genetics, Inc. This work was supported in part by the National Science Foundation under grant DBI-1004842. AP was supported by the National Science foundation under grant EPS-0903787 and by the Mississippi INBRE funded by grants from the National Center for Research Resources (5P20RR016476-11) and the National Institute of General Medical Sciences (8 P20 GM103476-11) from the National Institutes of Health.en_US
dc.identifier.doi10.1186/1477-7827-10-82
dc.identifier.issn1477-7827
dc.identifier.pmid22978562
dc.identifier.scopus2-s2.0-84866165341
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/1477-7827-10-82
dc.identifier.urihttps://hdl.handle.net/11468/18237
dc.identifier.volume10en_US
dc.identifier.wosWOS:000310752700001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherBiomed Central Ltden_US
dc.relation.ispartofReproductive Biology and Endocrinology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectUncompensatory Fertilityen_US
dc.subjectMammalianen_US
dc.subjectFertilizationen_US
dc.subjectEmbryonic Developmenten_US
dc.titleDynamics of microRNAs in bull spermatozoaen_US
dc.titleDynamics of microRNAs in bull spermatozoa
dc.typeArticleen_US

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