The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes

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dc.contributor.authorUzan, Serhat
dc.contributor.authorAcay, Hilal
dc.contributor.authorBaran, M. Firat
dc.contributor.authorBilden, Alican
dc.contributor.authorAygun, Husamettin
dc.date.accessioned2024-04-24T16:01:58Z
dc.date.available2024-04-24T16:01:58Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractIt is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC(50)value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver-Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.en_US
dc.identifier.doi10.1007/s00764-020-00030-3
dc.identifier.endpage269en_US
dc.identifier.issn0933-4173
dc.identifier.issn1789-0993
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85086788493
dc.identifier.scopusqualityQ3
dc.identifier.startpage263en_US
dc.identifier.urihttps://doi.org/10.1007/s00764-020-00030-3
dc.identifier.urihttps://hdl.handle.net/11468/14535
dc.identifier.volume33en_US
dc.identifier.wosWOS:000542927000001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer Heidelbergen_US
dc.relation.ispartofJpc-Journal of Planar Chromatography-Modern Tlc
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGlutathione-S-Transferaseen_US
dc.subjectMethylene Blueen_US
dc.subjectThin-Layer Chromatographyen_US
dc.subjectAffinity Chromatographyen_US
dc.titleThe interaction methylene blue and glutathione-S-transferase purified from human erythrocytesen_US
dc.titleThe interaction methylene blue and glutathione-S-transferase purified from human erythrocytes
dc.typeArticleen_US

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