The interaction methylene blue and glutathione-S-transferase purified from human erythrocytes

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Tarih

2020

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Springer Heidelberg

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

It is known that textile dyes have various risks on human health. Glutathione-S-transferase enzymes play a critical role in the detoxification of xenobiotics in living systems. This study aimed to examine the interaction of methylene blue with human erythrocyte glutathione-S-transferase purified in one step. Human erythrocyte glutathione-S-transferase was purified with approximately 750-fold purification and 30% efficiency by glutathione agarose affinity chromatography. The results showed that the enzyme was inhibited by methylene blue with an IC(50)value of 1.40 mmol/L. The Ki constant of methylene blue was 1.17 mmol/L. The Lineweaver-Burk graph of the methylene blue showed that the type of inhibition was compatible with mixed type inhibition. A new third spot was also detected by thin-layer chromatography. Furthermore, the cytotoxicity of methylene blue on human erythrocytes was evaluated and it was found that the haemolysis per cent of methylene blue on erythrocytes was approximately 14%.

Açıklama

Anahtar Kelimeler

Glutathione-S-Transferase, Methylene Blue, Thin-Layer Chromatography, Affinity Chromatography

Kaynak

Jpc-Journal of Planar Chromatography-Modern Tlc

WoS Q Değeri

Q4

Scopus Q Değeri

Q3

Cilt

33

Sayı

3

Künye