The prevalence and molecular typing of Clostridium perfringens in ground beef and sheep meats

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dc.contributor.authorGuran, Husnu Sahan
dc.contributor.authorVural, Aydin
dc.contributor.authorErkan, Mehmet Emin
dc.date.accessioned2024-04-24T15:59:42Z
dc.date.available2024-04-24T15:59:42Z
dc.date.issued2014
dc.departmentDicle Üniversitesien_US
dc.description.abstractThis study was aimed at the investigation of the prevalence of Clostridium perfringens in ground beef and sheep meats and to detect the cpa, cpb, etx, iA, cpe and cpb2 toxin genes by multiplex PCR. Obtained from retail markets and butchers, 100 ground meat samples, 50 of which were beef and 50 of which were sheep meat samples, were examined. Out of the 50 ground beef samples 48 (96 %) and out of the 50 ground sheep meat samples 44 (88 %) were contaminated with C. perfringens. According to microscopic examination and the results of biochemical tests, 262 positive isolates obtained from these samples were identified as C. perfringens. Multiplex PCR results demonstrated that, out of the 262 isolates, 203 (77.4 %) possessed only the cpa gene (type A), while 20 (7.6 %) carried the cpa and cpb2 toxin genes (type A-cpb2), and 6 (2.2 %) the cpa and cpe toxin genes (type A-cpe). Furthermore, it was determined that, out of the 262 C. perfringens isolates, 4 (1.5 %), 10 (3.8 %) and 19 (7.2 %) were of the types B, C and D, respectively.en_US
dc.description.sponsorshipDicle University [DUBAP/10-VF-83]en_US
dc.description.sponsorshipThe present study was supported by the Dicle University Research Fund (DUBAP/10-VF-83). We thank Senay Ercelik and Zulkuf Emre Papatya for their excellent laboratory assistance.en_US
dc.identifier.doi10.1007/s00003-014-0866-z
dc.identifier.endpage128en_US
dc.identifier.issn1661-5751
dc.identifier.issn1661-5867
dc.identifier.issue2en_US
dc.identifier.scopus2-s2.0-84901594097
dc.identifier.scopusqualityQ2
dc.identifier.startpage121en_US
dc.identifier.urihttps://doi.org/10.1007/s00003-014-0866-z
dc.identifier.urihttps://hdl.handle.net/11468/14218
dc.identifier.volume9en_US
dc.identifier.wosWOS:000336902800004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringer International Publishing Agen_US
dc.relation.ispartofJournal Fur Verbraucherschutz Und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectC. Perfringensen_US
dc.subjectGround Meaten_US
dc.subjectPrevalenceen_US
dc.subjectToxin Genesen_US
dc.subjectMultiplex Pcren_US
dc.titleThe prevalence and molecular typing of Clostridium perfringens in ground beef and sheep meatsen_US
dc.titleThe prevalence and molecular typing of Clostridium perfringens in ground beef and sheep meats
dc.typeArticleen_US

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