The prevalence and molecular typing of Clostridium perfringens in ground beef and sheep meats
[ X ]
Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Springer International Publishing Ag
Access Rights
info:eu-repo/semantics/closedAccess
Abstract
This study was aimed at the investigation of the prevalence of Clostridium perfringens in ground beef and sheep meats and to detect the cpa, cpb, etx, iA, cpe and cpb2 toxin genes by multiplex PCR. Obtained from retail markets and butchers, 100 ground meat samples, 50 of which were beef and 50 of which were sheep meat samples, were examined. Out of the 50 ground beef samples 48 (96 %) and out of the 50 ground sheep meat samples 44 (88 %) were contaminated with C. perfringens. According to microscopic examination and the results of biochemical tests, 262 positive isolates obtained from these samples were identified as C. perfringens. Multiplex PCR results demonstrated that, out of the 262 isolates, 203 (77.4 %) possessed only the cpa gene (type A), while 20 (7.6 %) carried the cpa and cpb2 toxin genes (type A-cpb2), and 6 (2.2 %) the cpa and cpe toxin genes (type A-cpe). Furthermore, it was determined that, out of the 262 C. perfringens isolates, 4 (1.5 %), 10 (3.8 %) and 19 (7.2 %) were of the types B, C and D, respectively.
Description
Keywords
C. Perfringens, Ground Meat, Prevalence, Toxin Genes, Multiplex Pcr
Journal or Series
Journal Fur Verbraucherschutz Und Lebensmittelsicherheit-Journal of Consumer Protection and Food Safety
WoS Q Value
Q3
Scopus Q Value
Q2
Volume
9
Issue
2
