Does the analysis based on a histological and immunohistochemical grading system in the model of BDL kidney allow the quantification of the degree of injury?

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dc.contributor.authorBüyükbayram, H
dc.contributor.authorÖztürk, H
dc.contributor.authorKara, IJ
dc.contributor.authorArslan, A
dc.date.accessioned2024-04-24T17:07:55Z
dc.date.available2024-04-24T17:07:55Z
dc.date.issued2004
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of this study is to evaluate histopathological findings induced by N-Omega-nitro-L-arginine methyl ester (L-NAME) and molsidomine (MOL) on the kidney of bile duct ligated rats. Forty Sprague-Dawley rats, each weighing 125 to 140 g, were included in the study. Extent of histological glomerular injury scores (GIS), arterial injury scores (AIS), and tubulointerstitial injury scores (TIS) in each animal were graded. Alpha-smooth muscle actin (alpha-SMA), tenascin, lectin (Ulex europaeus agglutinin-1), and vimentin were used to determine extent of the injury. The cholestasis was evidenced by a significant increase in the levels of serum total bilirubin in BDL rats (p<0.01). Malondialdeyde MDA levels increased by the bile duct ligation (BDL) to 12.10 +/- 0.45. This value was significantly higher than the other groups (p<0.01). Changes in the BDL kidney were marked at 7 days after surgery. GIS were observed to have the highest score, especially at juxtamedullary region in BDL/L-NAME rats, and AIS were also the highest score in this region. These observations were lower in BDL/MOL rats. There is a correlation between GIS and AIS scores (r=.2, p<.01). TIS revealed that BDL/L-NAME rats were significantly more damage than rats in the other groups (p<.001). MOL-treated rats showed considerably fewer lesions in the tubules and interstitium (p<.001). The tubular injuries observed in BDL and BDL/L-NAME rats were significantly attenuated by MOL treatment. Lectin was more and extensively stained in tubular epithelia of the BDL/L-NAME group than in the other (p<.05). Expression of tenascin in tubular epithelia was significantly higher in BDL and BDL/L-NAME as compared with controls (p<.01). Fibrous tissue was only observed in the BDL and BDL/L-NAME group. These areas were weakly stained with vimentin. alpha-SMA staining was more reduced in the L-NAME-treated arterioles than in BDL/MOL (p<.05). In conclusion, the analysis of cell injury based on a histological grading system in the model of BDL kidney allows the quantification of the degree of injury.en_US
dc.identifier.doi10.1081/JDI-200031727
dc.identifier.endpage495en_US
dc.identifier.issn0886-022X
dc.identifier.issn1525-6049
dc.identifier.issue5en_US
dc.identifier.pmid15526906
dc.identifier.scopus2-s2.0-5444262956
dc.identifier.scopusqualityQ2
dc.identifier.startpage487en_US
dc.identifier.urihttps://doi.org/10.1081/JDI-200031727
dc.identifier.urihttps://hdl.handle.net/11468/17097
dc.identifier.volume26en_US
dc.identifier.wosWOS:000224501400003
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherTaylor & Francis Ltden_US
dc.relation.ispartofRenal Failure
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBile Duct Obstructionen_US
dc.subjectKidneyen_US
dc.subjectMolsidomineen_US
dc.subjectL-Nameen_US
dc.subjectTenascinen_US
dc.subjectLectinen_US
dc.titleDoes the analysis based on a histological and immunohistochemical grading system in the model of BDL kidney allow the quantification of the degree of injury?en_US
dc.titleDoes the analysis based on a histological and immunohistochemical grading system in the model of BDL kidney allow the quantification of the degree of injury?
dc.typeArticleen_US

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