Expression pattern of microRNAs in ovine endometrium during the peri-implantation

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dc.contributor.authorKose, Mehmet
dc.contributor.authorHitit, Mustafa
dc.contributor.authorKaya, Mehmet Salih
dc.contributor.authorKirbas, Mesut
dc.contributor.authorDursun, Sukru
dc.contributor.authorAlak, Ilyas
dc.contributor.authorAtli, Mehmet Osman
dc.date.accessioned2024-04-24T16:18:29Z
dc.date.available2024-04-24T16:18:29Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractMicroRNA (miRNA), acting as the transcriptional regulator of gene expression, has been widely demonstrated to be involved in many biological functions, including embryo implantation and devel-opment. The objective of the current study was to illuminate the expression pattern of microRNAs (miRNAs) in the endometrium during the peri-implantation in ewes. Intercaruncular endometrial samples was obtained from a total of 24 ewes on days of 12 (pre-implantation, n = 4), 16 (implantation, n = 4) and 22 (post-implantation, n = 4) of pregnancy following mating, and on their corresponding days of 12 (n = 4), 16 (n = 4) and 22 (n = 4) of the estrous cycle. The miRNA profiles were examined in the endometrium by microarray technology. We detected 116 ovine specifics miRNAs in the endometrium. Of these, nineteen were differentially expressed in early pregnancy. Four miRNAs (oar-miR-370-3p, oar-miR-411b-5p, oar-miR-379-3p and oar-miR-411a-3p) that had the most differential fold change were confirmed by RT-qPCR in ovine endometrium. The differentially expressed miRNAs targeted a total of 315 genes, resulting in 39 GO terms in molecular function, 353 in biological process, and 17 in the cellular component. The construction of the PPI network of target genes established two functional modules mostly enriched in the innate immune system, toll receptor cascades in module 1, whereas genes in module 2 were associated with GMCSF-mediated signaling events, insulin pathway, and mTOR signaling pathway. Based on the results, we may imply that miRNAs modulate ovine endometrium during the peri-implantation.(c) 2022 Elsevier Inc. All rights reserved.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [214O643]en_US
dc.description.sponsorshipThe study wasfinancially funded by Scientific and Technological Research Council of Turkey (TUBITAK) projects (214O643) to MK.en_US
dc.identifier.doi10.1016/j.theriogenology.2022.07.015
dc.identifier.endpage46en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.pmid35944411
dc.identifier.scopus2-s2.0-85135911405
dc.identifier.scopusqualityQ1
dc.identifier.startpage35en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2022.07.015
dc.identifier.urihttps://hdl.handle.net/11468/16129
dc.identifier.volume191en_US
dc.identifier.wosWOS:000860162300005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectMicrornaen_US
dc.subjectExpressionen_US
dc.subjectOvineen_US
dc.subjectEndometriumen_US
dc.subjectEarly Pregnancyen_US
dc.titleExpression pattern of microRNAs in ovine endometrium during the peri-implantationen_US
dc.titleExpression pattern of microRNAs in ovine endometrium during the peri-implantation
dc.typeArticleen_US

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