Evaluating the glucantime concentration for the ex vivo glial cell model of antimony-resistant leishmania tropica amastigotes

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dc.authorid0000-0002-9645-7085en_US
dc.authorid0000-0003-0274-0427en_US
dc.authorid0000-0002-8845-1433en_US
dc.authorid0000-0003-3613-8741en_US
dc.authorid0000-0001-5744-0937en_US
dc.authorid0000-0003-4517-3223en_US
dc.contributor.authorZorbozan, Orçun
dc.contributor.authorEvren, Vedat
dc.contributor.authorHarman, Mehmet
dc.contributor.authorÖzbilgin, Ahmet
dc.contributor.authorYılmaz, Özlem Alkan
dc.contributor.authorTurgay, Nevin
dc.date.accessioned2023-10-24T12:50:45Z
dc.date.available2023-10-24T12:50:45Z
dc.date.issued2021en_US
dc.departmentDicle Üniversitesi, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Deri ve Zührevi Hastalıklar Ana Bilim Dalıen_US
dc.description.abstractObjective: Because the protocols used in the treatment of leishmaniasis can be toxic and have many limitations, such as the development of resistance against such protocols, new treatment options are needed, especially against resistant patients. Ex vivo models may be a good source for evaluating new drug options for patients with antimony-resistant parasites. This study aimed to evaluate the Glucantime concentration for our ex vivo glial cell amastigote model we had defined in previous work. Methods: We prepared the astroglial cell culture from brains of 2 to 3 day old neonatal Sprague-Dawley rats under sterile conditions by modifying McCarthy’s method. Four plates of cells were infected with antimony-resistant Leishmania tropica promastigotes. After 24 h of incubation, we added Glucantime to 3 plates with different concentrations. After 72 h, we removed the supernatant and then dried, fixed, and stained the plates with Giemsa to count the amastigotes in the glial cells. Results: We observed the amastigotes in glial cells in the control flask. Glial cells were ruined in flasks, which include 75 µg/ mL and 37.5 µg/mL Glucantime. The number of amastigotes per 100 glial cells was 116 for the flask with 7.5 µg/mL Glucantime concentration, while 487 for the control flask. Conclusion: We found that while high concentrations of Glucantime were toxic for glial cells, 7.5 µg/mL Glucantime concentration managed to reduce the number of Leishmania tropica amastigotes in glial cells.en_US
dc.identifier.citationZorbozan, O., Evren, V., Harman, M., Özbilgin, A., Yılmaz, Ö. A. ve Turgay, N. (2021). Evaluating the glucantime concentration for the ex vivo glial cell model of antimony-resistant leishmania tropica amastigotes. Türkiye Parazitoloji Dergisi, 45(4), 237-240.en_US
dc.identifier.doi10.4274/tpd.galenos.2021.57966
dc.identifier.endpage240en_US
dc.identifier.issn1300-6320
dc.identifier.issue4en_US
dc.identifier.pmid34889188
dc.identifier.scopus2-s2.0-85122647109
dc.identifier.scopusqualityQ3
dc.identifier.startpage237en_US
dc.identifier.trdizinid508858
dc.identifier.urihttps://cms.galenos.com.tr/Uploads/Article_49859/TPD-45-237-En.pdf
dc.identifier.urihttps://hdl.handle.net/11468/12919
dc.identifier.volume45en_US
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.indekslendigikaynakTR-Dizin
dc.institutionauthorHarman, Mehmet
dc.language.isoenen_US
dc.publisherGalenos Publishing Houseen_US
dc.relation.ispartofTürkiye Parazitoloji Dergisi
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAmastigoteen_US
dc.subjectAntimony resistanceen_US
dc.subjectEx vivoen_US
dc.subjectGlial cellen_US
dc.subjectLeishmania tropicaen_US
dc.titleEvaluating the glucantime concentration for the ex vivo glial cell model of antimony-resistant leishmania tropica amastigotesen_US
dc.titleEvaluating the glucantime concentration for the ex vivo glial cell model of antimony-resistant leishmania tropica amastigotes
dc.title.alternativeAntimon dirençli leishmania tropica amastigotlarının ex vivo glial hücre modeli için glukantim konsantrasyonunun değerlendirilmesien_US
dc.title.alternativeAntimon dirençli leishmania tropica amastigotlarının ex vivo glial hücre modeli için glukantim konsantrasyonunun değerlendirilmesi
dc.typeArticleen_US

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