Protective effects of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate in bladder ischemia-reperfusion injury in rats

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dc.contributor.authorYucel, Mehmet
dc.contributor.authorKucuk, Aysegul
dc.contributor.authorBayraktar, Aslihan Cavunt
dc.contributor.authorTosun, Murat
dc.contributor.authorYalcinkaya, Soner
dc.contributor.authorHatipoglu, Namik Kemal
dc.contributor.authorErkasap, Nilufer
dc.date.accessioned2024-04-24T16:02:17Z
dc.date.available2024-04-24T16:02:17Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe aim of the present study was to evaluate the protective effects of the NF-(DB)-B-0 inhibition with pyrrolidine-dithiocarbamate (PDTC) in ischemia-reperfusion (I/R) injury in the rat bladder. Twenty-four Sprague-Dawley male rats were divided into three groups. Group I; (n = 8) control, group II; (n = 8) I/R group; group III (n = 8) I/R and PDTC treatment. Superoxide dismutase (SOD), catalase (CAT), and gluatathione-S-transferase (GST) enzymes was studied in bladder tissue. Lipid peroxidation (as TBARS) levels in tissue homogenate were measured with thiobarbituric acid reaction. All the slides were stained with NF-(DB)-B-0, p53 and HSP60 immunohistochemistry for detection genome destruction and tissue stress, respectively. Our results show that the mean TBARS levels were significantly higher in group II (p < 0.05). The TBARS levels were significantly decreased in group III compared with the group II (p < 0.05). CAT, SOD and GST activities were decreased in group II, but these enzymes levels were significantly increased in group III according to the group II (p < 0.05). Under microscopic evaluation NF-(DB)-B-0 expression increased significantly in group II compared to the group I (p < 0.05) and then decreased in group III (p < 0.05). HSP60 and p53 expression in group II was increased significantly compared with group I. Under microscopic evaluation we detected that HSP60 and p53 expression was increased significantly in group II compared with group I. In group III PDTC administration was decreased the HSP60 and p53 expression, this difference was statistically significant (p < 0.05). The results of the present study have demonstrated that NF-(DB)-B-0 inhibition with PDTC protects and provides beneficial effects on ischemia/reperfusion stress related bladder tissue destruction.en_US
dc.identifier.doi10.1007/s11033-013-2676-2
dc.identifier.endpage5740en_US
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue10en_US
dc.identifier.pmid24057237
dc.identifier.scopus2-s2.0-84892745632
dc.identifier.scopusqualityQ2
dc.identifier.startpage5733en_US
dc.identifier.urihttps://doi.org/10.1007/s11033-013-2676-2
dc.identifier.urihttps://hdl.handle.net/11468/14729
dc.identifier.volume40en_US
dc.identifier.wosWOS:000325948400022
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntioxidanten_US
dc.subjectIschemia-Reperfusionen_US
dc.subjectNuclear Factor-Kappa Ben_US
dc.subjectPyrrolidine Dithiocarbamateen_US
dc.subjectBladder Ischemiaen_US
dc.titleProtective effects of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate in bladder ischemia-reperfusion injury in ratsen_US
dc.titleProtective effects of the nuclear factor kappa B inhibitor pyrrolidine dithiocarbamate in bladder ischemia-reperfusion injury in rats
dc.typeArticleen_US

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