Detection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers

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dc.contributor.authorAkdemir, Hulya
dc.contributor.authorSuzerer, Veysel
dc.contributor.authorTilkat, Engin
dc.contributor.authorOnay, Ahmet
dc.contributor.authorCiftci, Yelda Ozden
dc.date.accessioned2024-04-24T16:02:36Z
dc.date.available2024-04-24T16:02:36Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractDetermination of genetic stability of in vitro-grown plantlets is needed for safe and large-scale production of mature trees. In this study, genetic variation of long-term micropropagated mature pistachio developed through direct shoot bud regeneration using apical buds (protocol A) and in vitro-derived leaves (protocol B) was assessed via DNA-based molecular markers. Randomly amplified polymorphic DNA (RAPD), inter-simple sequence repeat (ISSR), and amplified fragment length polymorphism (AFLP) were employed, and the obtained PIC values from RAPD (0.226), ISSR (0.220), and AFLP (0.241) showed that micropropagation of pistachio for different periods of time resulted in reasonable polymorphism among donor plant and its 18 clones. Mantel's test showed a consistence polymorphism level between marker systems based on similarity matrices. In conclusion, this is the first study on occurrence of genetic variability in long-term micropropagated mature pistachio plantlets. The obtained results clearly indicated that different marker approaches used in this study are reliable for assessing tissue culture-induced variations in long-term cultured pistachio plantlets.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [KBAG 209 T030]en_US
dc.description.sponsorshipThe authors are grateful to MSc. Emrah Kirdok for helping to statistical analysis. This research was funded by a grant no. KBAG 209 T030 from The Scientific and Technological Research Council of Turkey (TUBITAK).en_US
dc.identifier.doi10.1007/s12010-016-2168-7
dc.identifier.endpage1312en_US
dc.identifier.issn0273-2289
dc.identifier.issn1559-0291
dc.identifier.issue7en_US
dc.identifier.pmid27318708
dc.identifier.scopus2-s2.0-84975129428
dc.identifier.scopusqualityQ2
dc.identifier.startpage1301en_US
dc.identifier.urihttps://doi.org/10.1007/s12010-016-2168-7
dc.identifier.urihttps://hdl.handle.net/11468/14853
dc.identifier.volume180en_US
dc.identifier.wosWOS:000389891800004
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPistacia Veraen_US
dc.subjectTissue Culture-Induced Variationen_US
dc.subjectRapden_US
dc.subjectIssren_US
dc.subjectAflpen_US
dc.titleDetection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markersen_US
dc.titleDetection of Variation in Long-Term Micropropagated Mature Pistachio via DNA-Based Molecular Markers
dc.typeArticleen_US

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