Is Apoptosis a Regulatory Mechanism During Early Canine Pregnancy?

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dc.contributor.authorSchaefer-Somi, S.
dc.contributor.authorSabitzer, S.
dc.contributor.authorKlein, D.
dc.contributor.authorTomaszewski, C.
dc.contributor.authorKanca, H.
dc.contributor.authorBeceriklisoy, H. B.
dc.contributor.authorKucukaslan, I.
dc.date.accessioned2024-04-24T17:11:50Z
dc.date.available2024-04-24T17:11:50Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractContents Fas is a membrane-bound protein which upon activation causes programmed cell death. Fas ligand (FasL) binds Fas on target cells. Both these factors are known to regulate apoptosis at implantation in different species and thus might be involved in the regulation of implantation in dogs. The aim of the study was to assess the expression of Fas and FasL in canine uterine tissue throughout pregnancy as well as in pre-implantation embryos using RT-PCR and RT-qPCR. Uterine tissues was collected from of 21 healthy pregnant bitches (group I: days 1012, n=5; group II: days 1825, n=6; group III: days 2845, n=6) and from 4 non-pregnant bitches (controls: days 1012). Pregnancy stage was determined by days after mating, that is, 23days after ovulation as determined by vaginal cytology and progesterone measurement. After ovariohysterectomy, uteri from group I bitches were flushed with PBS and the embryos washed and stored frozen at -80 degrees. Tissues from the other groups were taken from the implantation and placentation sites, respectively, covered with Tissue Tek (R) and frozen at -80 degrees. Extraction of RNA was performed with Trizol Reagent and RT-qPCR using SYBR green probes. In pre-implantation embryos, only FasL but not Fas could be detected. In all tissues from pregnant and non-pregnant bitches, both parameters were detectable. Before implantation (group I) expression of FasL resembled that of non-pregnant bitches in early dioestrus and decreased significantly during implantation and thereafter (p<0.05). Expression of Fas did not change significantly until day 45. The relative expression of Fas exceeded that of FasL at each stage investigated, which is comparable to observations of other species; however, high standard deviations indicate high individual differences. These preliminary results point towards a regulatory function of the Fas/FasL system during early canine pregnancy.en_US
dc.identifier.doi10.1111/rda.12063
dc.identifier.endpage172en_US
dc.identifier.issn0936-6768
dc.identifier.pmid23279491
dc.identifier.scopus2-s2.0-84871695479
dc.identifier.scopusqualityQ2
dc.identifier.startpage169en_US
dc.identifier.urihttps://doi.org/10.1111/rda.12063
dc.identifier.urihttps://hdl.handle.net/11468/17743
dc.identifier.volume47en_US
dc.identifier.wosWOS:000312888300038
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherWiley-Blackwellen_US
dc.relation.ispartofReproduction in Domestic Animals
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keyword]en_US
dc.titleIs Apoptosis a Regulatory Mechanism During Early Canine Pregnancy?en_US
dc.titleIs Apoptosis a Regulatory Mechanism During Early Canine Pregnancy?
dc.typeArticleen_US

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