Anoxybacillus sp AH1, an ?-amylase-producing thermophilic bacterium isolated from Dargecit hot spring

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dc.contributor.authorAcer, Omer
dc.contributor.authorPirinccioglu, Hemse
dc.contributor.authorBekler, Fatma Matpan
dc.contributor.authorGul-Guven, Reyhan
dc.contributor.authorGuven, Kemal
dc.date.accessioned2024-04-24T17:18:00Z
dc.date.available2024-04-24T17:18:00Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe present study was conducted to isolate alpha-amylase-producing thermophilic bacteria from Dargecit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60 degrees C and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular a-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased a-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl2 up to 100 mM.en_US
dc.identifier.doi10.1515/biolog-2015-0111
dc.identifier.endpage862en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue7en_US
dc.identifier.scopus2-s2.0-84940824417
dc.identifier.scopusqualityQ2
dc.identifier.startpage853en_US
dc.identifier.urihttps://doi.org/10.1515/biolog-2015-0111
dc.identifier.urihttps://hdl.handle.net/11468/18526
dc.identifier.volume70en_US
dc.identifier.wosWOS:000360124200001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologia
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnoxybacillusen_US
dc.subjectIsolation And Characterisationen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectThermophilic Bacteriaen_US
dc.titleAnoxybacillus sp AH1, an ?-amylase-producing thermophilic bacterium isolated from Dargecit hot springen_US
dc.titleAnoxybacillus sp AH1, an ?-amylase-producing thermophilic bacterium isolated from Dargecit hot spring
dc.typeArticleen_US

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