Anoxybacillus sp AH1, an ?-amylase-producing thermophilic bacterium isolated from Dargecit hot spring
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Tarih
2015
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Springer
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The present study was conducted to isolate alpha-amylase-producing thermophilic bacteria from Dargecit hot springs in Turkey. The morphological, biochemical and physiological characterisation, as well as genetic analysis by 16S rRNA sequences indicated that the isolated strain AH1 was a member of Anoxybacillus genus. The strain was aerobe, Gram-positive and spore-forming rod, exhibiting optimum growth temperature and pH of 60 degrees C and 7.0-7.5, respectively. Optimization of growth medium and enzyme assay conditions for extracellular a-amylase production by the novel thermophilic Anoxybacillus sp. AH1 were carried out in many different media containing a variety of carbon and nitrogen sources. Among various carbon and nitrogen sources, peptone (2054.1 U/mL) at 1% and maltose (1862.9 U/mL) at 0.5% increased a-amylase activity, compared to controls. Moreover, a high enzyme production was observed with potato starch at 0.5% and 1% (2668.4 U/mL and 3627 U/mL, respectively), as well as with 1% soluble starch (2051.9 U/mL). The enzyme activity was found to be rather high in the presence of CaCl2 up to 100 mM.
Açıklama
Anahtar Kelimeler
Anoxybacillus, Isolation And Characterisation, Alpha-Amylase, Thermophilic Bacteria
Kaynak
Biologia
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
70
Sayı
7
