Protective effects of carvacrol and pomegranate against methotrexate-induced intestinal damage in rats

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dc.contributor.authorTurkcu, Gul
dc.contributor.authorAlabalik, Ulas
dc.contributor.authorKeles, Ayse Nur
dc.contributor.authorBozkurt, Mehtap
dc.contributor.authorIbiloglu, Ibrahim
dc.contributor.authorFirat, Ugur
dc.contributor.authorBuyukbayram, Huseyin
dc.date.accessioned2024-04-24T17:37:52Z
dc.date.available2024-04-24T17:37:52Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractObjective: The purpose of this experimental study was to evaluate the efficacy of carvacrol (CVR) and pomegranate (PMG) against methotrexate (MTX)-induced intestinal damage using histopathological and immunohistochemical techniques. Methods: Thirty-two male Sprague-Dawley rats, weighing 195-250 g, were divided into four groups: control, MTX treatment alone, MTX plus CVR and MTX plus PMG. A single dose of CVR (73 mg/kg) was administered intraperitoneally to group III on the first day of the experiment, PMG (225 mg/kg/day) was administered orogastrically (with a gavage needle) once daily for 7 days and a single dose of MTX (20 mg/kg) was administered intraperitoneally on the second day of the experiment. Intestinal tissues were obtained on 8th day, and examined for villus damage, crypt damage, and inflammation. Ki-67 and Caspase 3 staining was used for immunohistochemical evaluation. Results: MTX treatment induced villus shortening and fusion, epithelial atrophy, crypt loss, inflammatory infiltrate in the lamina propria, and goblet cell depletion. The CVR and PMG decreased the severity of intestinal damage caused by MTX treatment. In the MTX-received group, significant inflammatory cell infiltration was observed in the lamina propria. Compared to the MTX-received group, the PMG and CVR groups showed less villus and crypt damage and less inflammation in the lamina propria. Fewer Ki-67 positive cells were observed in the crypts of the MTX-received groups compared to the control group. There were more Ki-67 positive cells in the CVR and PMG groups compared to MTX group. The MTX-received group exhibited more caspase-3 positive cells than the control group, and the number of caspase-3 positive cells were decreased in the CVR and PMG treated groups. Conclusion: This study is the first to show that PMG and CVR decrease MTX-related damage and apoptotic activity in intestinal tissue.en_US
dc.description.sponsorshipScientific Research Fund of Dicle University; Dicle University DUBAPen_US
dc.description.sponsorshipThis study was supported by Scientific Research Fund of Dicle University. The authors have no financial interest in any of the products mentioned in the manuscript. We are grateful to Dicle University DUBAP for their sponsorship about English editing of this manuscript.en_US
dc.identifier.endpage15481en_US
dc.identifier.issn1940-5901
dc.identifier.issue9en_US
dc.identifier.pmid26629037
dc.identifier.scopus2-s2.0-84946593977
dc.identifier.scopusqualityN/A
dc.identifier.startpage15474en_US
dc.identifier.urihttps://hdl.handle.net/11468/21223
dc.identifier.volume8en_US
dc.identifier.wosWOS:000367911200050
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherE-Century Publishing Corpen_US
dc.relation.ispartofInternational Journal of Clinical and Experimental Medicine
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCarvacrolen_US
dc.subjectIntestinal Damageen_US
dc.subjectMethotrexateen_US
dc.subjectPomegranateen_US
dc.titleProtective effects of carvacrol and pomegranate against methotrexate-induced intestinal damage in ratsen_US
dc.titleProtective effects of carvacrol and pomegranate against methotrexate-induced intestinal damage in rats
dc.typeArticleen_US

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