Adapted T Cell Interferon-Gamma Release Assay for the Diagnosis of Pleural Tuberculosis

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dc.contributor.authorAtes, Gungor
dc.contributor.authorYildiz, Tekin
dc.contributor.authorOrtakoylu, Mediha Gonenc
dc.contributor.authorOzekinci, Tuncer
dc.contributor.authorErturk, Baykal
dc.contributor.authorAkyildiz, Levent
dc.contributor.authorCaglar, Emel
dc.date.accessioned2024-04-24T17:14:28Z
dc.date.available2024-04-24T17:14:28Z
dc.date.issued2011
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: Better and more rapid tests are needed for the diagnosis of tuberculous pleural effusion (TPE), given the known limitations of conventional diagnostic tests. Objectives: To estimate diagnostic accuracy of the QuantiFERON-TB Gold In-Tube (QFT-GIT) test (and its components) using data-derived cutoffs in pleural fluid. Methods: The QFT-GIT test was performed on whole blood and pleural fluid from 43 patients with TPE and 29 control subjects (non-TPE). To achieve the objective, QFT-GIT test, estimating likelihood ratios and receiver operating curve analysis were performed. Results: The sensitivity and specificity using the QFT-GIT for the diagnosis of TPE were 48.8% and 79.3%, respectively, in pleural fluid. The best cutoff points for tuberculosis (TB) antigen, nil and TB antigen minus nil results were estimated at 0.70, 0.90 and 0.30 IU/ml, respectively. Area under the curve of TB antigen IFN-gamma response was 0.86 (CI: 0.76-0.93), nil tube was 0.80 (CI: 0.69-0.89) and TB antigen minus nil tube was 0.82 (CI: 0.72-0.90). When the best cutoff scores of the nil tubes were set at this value, the results of a likelihood ratio of a positive and a negative test were 9.44 (7.4-12.0) and 0.37 (0.09-1.5), respectively. The percentages of indeterminate results in pleural fluid among the TPE cases were 42% (most of them caused by high nil IFN-gamma values) using the QFT-GIT test. Conclusion: QFT-GIT test or its components have poor accuracy in the diagnosis of TPE, largely because of a high number of indeterminate results due to high background IFN-gamma production in the TPE. Copyright (C) 2011 S. Karger AG, Baselen_US
dc.description.sponsorshipUniversity of Dicleen_US
dc.description.sponsorshipThis study was funded by a grant from the University of Dicle.en_US
dc.identifier.doi10.1159/000323184
dc.identifier.endpage357en_US
dc.identifier.issn0025-7931
dc.identifier.issue4en_US
dc.identifier.pmid21293103
dc.identifier.scopus2-s2.0-80052964062
dc.identifier.scopusqualityQ1
dc.identifier.startpage351en_US
dc.identifier.urihttps://doi.org/10.1159/000323184
dc.identifier.urihttps://hdl.handle.net/11468/17963
dc.identifier.volume82en_US
dc.identifier.wosWOS:000294901500007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherKargeren_US
dc.relation.ispartofRespiration
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPleurisyen_US
dc.subjectQuantiferonen_US
dc.subjectT Cell Ifn-Gamma Release Assayen_US
dc.subjectTuberculosisen_US
dc.titleAdapted T Cell Interferon-Gamma Release Assay for the Diagnosis of Pleural Tuberculosisen_US
dc.titleAdapted T Cell Interferon-Gamma Release Assay for the Diagnosis of Pleural Tuberculosis
dc.typeArticleen_US

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