Geobacillus subterraneus subsp aromaticivorans subsp nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey

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dc.contributor.authorPoli, Annarita
dc.contributor.authorGuven, Kemal
dc.contributor.authorRomano, Ida
dc.contributor.authorPirinccioglu, Hamsi
dc.contributor.authorGuven, Reyhan Gul
dc.contributor.authorEuzeby, Jean Paul Marie
dc.contributor.authorMatpan, Fatma
dc.date.accessioned2024-04-24T17:18:46Z
dc.date.available2024-04-24T17:18:46Z
dc.date.issued2012
dc.departmentDicle Üniversitesien_US
dc.description.abstractA new thermophilic spore-forming strain Ge1(T) was isolated from the Guclukonak hot spring in Sirnak, Turkey. The strain was identified by using a polyphasic taxonomic approach. Strain Ge1(T) was Gram-positive, spore-forming, alkaliphilic rod-shaped, motile, occurring in pairs or filamentous. Growth was observed between 30 and 65 degrees C (optimum 60 degrees C) and at pH 5.5-10.0 (optimum pH 9.0). It was capable of utilizing starch, growth was observed at 0-3% NaCl (w/v) and was positive for catalase and urease. The major cellular fatty acids were iso-C-15:0 and iso-C-17:0, and the predominant lipoquinone found was menaquinone MK7 type. The DNA G+C content of the genomic DNA of strain Ge1(T) was 52.0%. Comparative 16S rRNA gene sequence studies showed that the isolate belonged to the genus Geobacillus. The DNA-DNA hybridization mean values between the representative strain Ge1(T) and the closely related species G. subterraneus, G. thermodenitrificans, G. thermocatenulatus, G. vulcani and G. thermoleovorans were 69.3%, 57%, 37%, 27% and 26%, respectively. The results of DNA-DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Ge1(T). Based on these results, we propose assigning a novel subspecies of Geobacillus subterraneus, to be named as Geobacillus subterraneus subsp. aromaticivorans subsp. nov. with the type strain Ge1(T) (DSM 23066 (T)= CIP 110341(T)).en_US
dc.description.sponsorshipIndustrial Research Project [PON01_01966 ENERBIOCHEM]; Scientific Research Commission of Dicle University-Diyarbakir [DUAPK-09-FF-50, DUAPK-09-FF-51]en_US
dc.description.sponsorshipThe paper was partially supported by Industrial Research Project PON01_01966 ENERBIOCHEM and by the Scientific Research Commission of Dicle University-Diyarbakir (Projects DUAPK-09-FF-50 and DUAPK-09-FF-51). The authors thank Eduardo Pagnotta for technical assistance.en_US
dc.identifier.doi10.2323/jgam.58.437
dc.identifier.endpage446en_US
dc.identifier.issn0022-1260
dc.identifier.issn1349-8037
dc.identifier.issue6en_US
dc.identifier.pmid23337579
dc.identifier.scopus2-s2.0-84873831384
dc.identifier.scopusqualityQ3
dc.identifier.startpage437en_US
dc.identifier.urihttps://doi.org/10.2323/jgam.58.437
dc.identifier.urihttps://hdl.handle.net/11468/18902
dc.identifier.volume58en_US
dc.identifier.wosWOS:000313755900005
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherMicrobiol Res Foundationen_US
dc.relation.ispartofJournal of General and Applied Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDna-Dna Hybridization Analysisen_US
dc.subjectGeobacillus Subterraneus Subsp Aromaticivorans Subsp Nov.en_US
dc.subjectLipidsen_US
dc.subjectTaxonomyen_US
dc.subjectThermophileen_US
dc.titleGeobacillus subterraneus subsp aromaticivorans subsp nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkeyen_US
dc.titleGeobacillus subterraneus subsp aromaticivorans subsp nov., a novel thermophilic and alkaliphilic bacterium isolated from a hot spring in Sirnak, Turkey
dc.typeArticleen_US

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