Purification and characterization of polyphenol oxidase from corn tassel

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dc.contributor.authorGuven, R. Gul
dc.contributor.authorAslan, N.
dc.contributor.authorGuven, K.
dc.contributor.authorBekler, F. Matpan
dc.contributor.authorAcer, O.
dc.date.accessioned2024-04-24T17:17:56Z
dc.date.available2024-04-24T17:17:56Z
dc.date.issued2016
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn this study, polyphenol oxidase (PPO) from corn tassel was extracted and partially purified through (NH4)(2)SO4 precipitation and gel filtration chromatography. Optimal temperatures for subsrates catechol and 4-methyl catechol were 40 degrees C and 30 degrees C, respectively. The optimal pH values were 8.0 for catechol and 6.0 for 4-methyl catechol. Catechol was the most suitible substrate (Km: 3.48 mM, Vmax: 1.0 Abs./min.). The moleculer mass of PPO was determined as 158 kDa. In this work, sodium azide, ethylenediaminetetraacetic acid (EDTA) and sodium dodecyl sulfate (SDS) were found to inhibit the enzyme activity as 26.6 %, 22.2 % and 12.2 % ratio, respectively. Besides, the effects of carbohydrates such as sucrose, fructose, ribose and glucose on PPO activity were investigated. The enzyme was found to be activated 17 % by fructose and ribose, 16 % by glucose and 4 % by sucrose.en_US
dc.identifier.doi10.14715/cmb/2016.62.13.2
dc.identifier.endpage11en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue13en_US
dc.identifier.pmid28040055
dc.identifier.scopus2-s2.0-85012028845
dc.identifier.scopusqualityQ4
dc.identifier.startpage6en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2016.62.13.2
dc.identifier.urihttps://hdl.handle.net/11468/18466
dc.identifier.volume62en_US
dc.identifier.wosWOS:000396122400002
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCorn Tasselen_US
dc.subjectPolyphenol Oxidaseen_US
dc.subjectCharacterisationen_US
dc.subjectSubstrate Specifityen_US
dc.subjectInhibitionen_US
dc.titlePurification and characterization of polyphenol oxidase from corn tasselen_US
dc.titlePurification and characterization of polyphenol oxidase from corn tassel
dc.typeArticleen_US

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