Effects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS

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dc.contributor.authorKaplan, Ibrahim
dc.contributor.authorYuksel, Hatice
dc.contributor.authorEvliyaoglu, Osman
dc.contributor.authorBasarali, M. Kemal
dc.contributor.authorToprak, Gulten
dc.contributor.authorColpan, Leyla
dc.contributor.authorSen, Velat
dc.date.accessioned2024-04-24T17:12:15Z
dc.date.available2024-04-24T17:12:15Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractTacrolimus and cyclosporine A are immunosuppressant drugs with narrow therapeutic windows. The aim of this study was to investigate the stability of tacrolimus and cyclosporin A levels in whole blood samples under different storage conditions. Whole blood samples were obtained from 15 patients receiving tacrolimus and 15 patients receiving cyclosporine A. Samples were immediately analyzed and then stored at different conditions (room temperature (24 degrees C-26 degrees C) for 24 hours, + 4 degrees C for 24 and 48 hours, and -20 degrees C for one month) and then analyzed again. For tacrolimus, there was a significant difference between samples analyzed immediately and those kept 24 hours at room temperature (P = 0.005) (percent change 32.89%). However, there were no significant differences between the other groups. For cyclosporine A, there was a significant difference between samples analyzed immediately and those kept 24 hours (P = 0.003) (percent change 19.47%) and 48 hours (P = 0.002) (percent change 15.38%) at + 4 degrees C and those kept 24 hours at room temperature (P = 0.011) (percent change 9.71%). Samples of tacrolimus should be analyzed immediately or stored at either + 4 degrees C or -20 degrees C, while samples of cyclosporine A should be analyzed immediately or stored at -20 degrees C.en_US
dc.description.sponsorshipDicle University DUBAPen_US
dc.description.sponsorshipThe authors are grateful to Dicle University DUBAP for their sponsorship of English editing of this paper.en_US
dc.identifier.doi10.1155/2015/956389
dc.identifier.issn1687-8760
dc.identifier.issn1687-8779
dc.identifier.pmid25949240
dc.identifier.scopus2-s2.0-84928564854
dc.identifier.scopusqualityQ3
dc.identifier.urihttps://doi.org/10.1155/2015/956389
dc.identifier.urihttps://hdl.handle.net/11468/17907
dc.identifier.volume2015en_US
dc.identifier.wosWOS:000353144500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherHindawi Ltden_US
dc.relation.ispartofInternational Journal of Analytical Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject[No Keyword]en_US
dc.titleEffects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MSen_US
dc.titleEffects of Storage Temperature and Time on Stability of Serum Tacrolimus and Cyclosporine A Levels in Whole Blood by LC-MS/MS
dc.typeArticleen_US

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