The protective effects of caffeic acid phenethyl ester in isoniazid and ethambutol-induced ocular toxicity of rats

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dc.contributor.authorSahin, Alparslan
dc.contributor.authorCingu, Abdullah Kursat
dc.contributor.authorKaya, Savas
dc.contributor.authorTurkcu, Gul
dc.contributor.authorAri, Seyhmus
dc.contributor.authorEvliyaoglu, Osman
dc.contributor.authorCinar, Yasin
dc.date.accessioned2024-04-24T17:20:34Z
dc.date.available2024-04-24T17:20:34Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractPurpose: This study intended to examine the effect of caffeic acid phenethyl ester (CAPE) on isoniazid (INH) and/or ethambutol (ETM)-induced retina and optic nerve toxicity in a rat model. Methods: This study included eight groups, each containing 10 rats. The groups were Control, INH, ETM, CAPE, INH+CAPE, ETM+CAPE, INH+ETM and INH+ETM+CAPE. Rats were given orally 50 mg/kg/d of INH and 50 mg/kg/d of ETM in tap water for 30 d. 10 mu mol/kg of CAPE were intraperitoneally injected for 30 d. The first dose of CAPE was given 24 h before the INH and ETM treatment and continued until sacrifice. Control group was given only tap water for 30 d. Rats were anaesthetized and sacrificed on the 30th day of experiment. Superoxide dismutase (SOD) activities, malondialdehyde (MDA), total anti-oxidant status (TAS), total oxidant status (TOS) were measured on the dissected and excised retina and optic nerve samples. Fellow eyes were used for histopathologic evaluation and the retinal ganglion cell (RGC) count. In addition, CAPE, INH and ETM interaction with SOD isoforms were calculated in silico. Results: The SOD activity and TAS levels were found significantly higher in CAPE-treated groups compared to INH and/or ETM-treated groups (p<0.0001). But the MDA, and TOS levels were significantly lower in CAPE-treated groups (p<0.0001). The mean RGC count is significantly decreased in INH, ETM and INH+ETM groups compared with INH+CAPE, ETM+CAPE and INH+ETM+CAPE groups, respectively (p values 0.001, 0.042, and 0.001 respectively). Besides, in silico calculations showed that binding affinity of CAPE to SOD isotypes was higher than that of INH and ETM. Conclusion: This study demonstrates that CAPE treatment may decrease the oxidative stress in the retina and optic nerve of INH- and ETM-treated rats and may prevent RGC loss. As an underlying mechanism, CAPE and SOD interaction seems crucial for alleviation of ocular oxidative stress and RGCs toxicity.en_US
dc.description.sponsorshipDicle University Scientific Research Project Committee [10-TF-66]en_US
dc.description.sponsorshipThis study is supported by Dicle University Scientific Research Project Committee (Project number is 10-TF-66).en_US
dc.identifier.doi10.3109/15569527.2012.759958
dc.identifier.endpage233en_US
dc.identifier.issn1556-9527
dc.identifier.issue3en_US
dc.identifier.pmid23351037
dc.identifier.scopus2-s2.0-84883041875
dc.identifier.scopusqualityQ3
dc.identifier.startpage228en_US
dc.identifier.urihttps://doi.org/10.3109/15569527.2012.759958
dc.identifier.urihttps://hdl.handle.net/11468/19139
dc.identifier.volume32en_US
dc.identifier.wosWOS:000323490400007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherInforma Healthcareen_US
dc.relation.ispartofCutaneous and Ocular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaffeic Acid Phenethyl Esteren_US
dc.subjectEthambutolen_US
dc.subjectIsoniaziden_US
dc.subjectNeuropathyen_US
dc.subjectOptic Nerveen_US
dc.subjectOxidative Stressen_US
dc.subjectRetinaen_US
dc.subjectRetinopathyen_US
dc.subjectToxicityen_US
dc.titleThe protective effects of caffeic acid phenethyl ester in isoniazid and ethambutol-induced ocular toxicity of ratsen_US
dc.titleThe protective effects of caffeic acid phenethyl ester in isoniazid and ethambutol-induced ocular toxicity of rats
dc.typeArticleen_US

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