Development of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones

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dc.contributor.authorAral, Hayriye
dc.contributor.authorAral, Tarik
dc.contributor.authorZiyadanogullari, Berrin
dc.contributor.authorZiyadanogullari, Recep
dc.date.accessioned2024-04-24T16:18:25Z
dc.date.available2024-04-24T16:18:25Z
dc.date.issued2013
dc.departmentDicle Üniversitesien_US
dc.description.abstractA novel amide-bonded silica stationary phase was prepared starting from N-Boc-phenylalanine, cyclohexylamine and spherical silica gel (4 mu m, 60 angstrom). The amide ligand was synthesised with high yield. The resulting amide bonded stationary phase was characterised by SEM, IR and elemental analysis. The resulting selector bearing a polar amide group is used for the reversed-phase chromatography separation of different classes of thirteen phytohormones (plant hormones). The chromatographic behaviours of these analytes on the amide-silica stationary phase were compared with those of RP-C18 column under same conditions. The effects of different separation conditions, such as mobile phase, pH value, flow rate and temperature, on the separation and retention behaviours of the 13 phytohormones in this system were studied. The optimum separation was achieved using reversed-phase HPLC gradient elution with an aqueous mobile phase containing pH = 6.85 potassium phosphate buffer (20 mM) and acetonitrile with a 22 degrees C column temperature. Under these experimental conditions, the 12 phytohormones could be separated and detected at 230 or 270 nm within 26 min. (C) 2013 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipResearch Project Council of Dicle University (DUBAP) [10-FF-29]en_US
dc.description.sponsorshipThe authors gratefully acknowledge the financial support provided by the Research Project Council of Dicle University (DUBAP) (Project no. 10-FF-29).en_US
dc.identifier.doi10.1016/j.talanta.2013.05.007
dc.identifier.endpage163en_US
dc.identifier.issn0039-9140
dc.identifier.issn1873-3573
dc.identifier.pmid24148387
dc.identifier.scopus2-s2.0-84878547481
dc.identifier.scopusqualityQ1
dc.identifier.startpage155en_US
dc.identifier.urihttps://doi.org/10.1016/j.talanta.2013.05.007
dc.identifier.urihttps://hdl.handle.net/11468/16080
dc.identifier.volume116en_US
dc.identifier.wosWOS:000328176000024
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofTalanta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAmide Stationary Phaseen_US
dc.subjectAmide Embeddeden_US
dc.subjectPlant Growth Regulatoren_US
dc.subjectHerbicidesen_US
dc.subjectHplc Separationen_US
dc.subjectReversed-Phaseen_US
dc.titleDevelopment of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormonesen_US
dc.titleDevelopment of a novel amide-silica stationary phase for the reversed-phase HPLC separation of different classes of phytohormones
dc.typeArticleen_US

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