Production of a-amylase by thermotolerant Bacillus subtilis in the presence of some carbon, nitrogen containing compounds and surfactants
[ X ]
Tarih
2003
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Inst Microbiologia
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The effect of some carbon, nitrogen sources and surfactants on the alpha-amylase production of Bacillus subtilis isolated from hot-spring water was investigated. Galactose, glucose, xylose, sucrose. fructose or corn starch were used as a carbon sources for production of alpha-amylase. Methionine, glycine, aspartic acid, lysine, (NH4)(2)SO4, NH4Cl, (NH4)(2)S2O8 and NH4NO3 were used as a organic and inorganic nitrogen sources, respectively. The higher alpha-amylase production was determined in corn starch, Sucrose, fructose and galactose compared to other sugars and with lysine and methionine than compared with other organic nitrogen sources. Nitrate and aspartic acid are clearly not as good a nitrogen Source for alpha-amylase production. When Triton X 100, sodium dodecyl Sulphate (SDS) and Tween 40 were employed in growth medium, although bacterial growth was found high, enzyme production was not detectable. Soluble starch and yeast extract were used in order to evaluate the influence of the medium composition on the alpha-amylase production instead of sole carbon and nitrogen Sources. Maximum enzyme production was found in 3.5 % and 1.5% soluble starch and yeast extract growth media, respectively.
Açıklama
Anahtar Kelimeler
Bacillus Subtilis, Alpha-Amylase, Carbon And Nitrogen Sources
Kaynak
Annals of Microbiology
WoS Q Değeri
Q4
Scopus Q Değeri
Q2
Cilt
53
Sayı
3
