Production of a-amylase by thermotolerant Bacillus subtilis in the presence of some carbon, nitrogen containing compounds and surfactants

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dc.contributor.authorBaysal, Z
dc.contributor.authorUyar, F
dc.contributor.authorAytekin, Ç
dc.date.accessioned2024-04-24T17:37:50Z
dc.date.available2024-04-24T17:37:50Z
dc.date.issued2003
dc.departmentDicle Üniversitesien_US
dc.description.abstractThe effect of some carbon, nitrogen sources and surfactants on the alpha-amylase production of Bacillus subtilis isolated from hot-spring water was investigated. Galactose, glucose, xylose, sucrose. fructose or corn starch were used as a carbon sources for production of alpha-amylase. Methionine, glycine, aspartic acid, lysine, (NH4)(2)SO4, NH4Cl, (NH4)(2)S2O8 and NH4NO3 were used as a organic and inorganic nitrogen sources, respectively. The higher alpha-amylase production was determined in corn starch, Sucrose, fructose and galactose compared to other sugars and with lysine and methionine than compared with other organic nitrogen sources. Nitrate and aspartic acid are clearly not as good a nitrogen Source for alpha-amylase production. When Triton X 100, sodium dodecyl Sulphate (SDS) and Tween 40 were employed in growth medium, although bacterial growth was found high, enzyme production was not detectable. Soluble starch and yeast extract were used in order to evaluate the influence of the medium composition on the alpha-amylase production instead of sole carbon and nitrogen Sources. Maximum enzyme production was found in 3.5 % and 1.5% soluble starch and yeast extract growth media, respectively.en_US
dc.identifier.endpage328en_US
dc.identifier.issn1590-4261
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-0346474184
dc.identifier.scopusqualityQ2
dc.identifier.startpage323en_US
dc.identifier.urihttps://hdl.handle.net/11468/21206
dc.identifier.volume53en_US
dc.identifier.wosWOS:000186028200007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoenen_US
dc.publisherInst Microbiologiaen_US
dc.relation.ispartofAnnals of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBacillus Subtilisen_US
dc.subjectAlpha-Amylaseen_US
dc.subjectCarbon And Nitrogen Sourcesen_US
dc.titleProduction of a-amylase by thermotolerant Bacillus subtilis in the presence of some carbon, nitrogen containing compounds and surfactantsen_US
dc.titleProduction of a-amylase by thermotolerant Bacillus subtilis in the presence of some carbon, nitrogen containing compounds and surfactants
dc.typeArticleen_US

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