Trolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats

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dc.contributor.authorSeker, Ugur
dc.contributor.authorNergiz, Yusuf
dc.contributor.authorAktas, Ayfer
dc.contributor.authorAkkus, Murat
dc.contributor.authorOzmen, Mehmet Ferit
dc.contributor.authorUyar, Emre
dc.contributor.authorSoker, Sevda
dc.date.accessioned2024-04-24T16:15:21Z
dc.date.available2024-04-24T16:15:21Z
dc.date.issued2020
dc.departmentDicle Üniversitesien_US
dc.description.abstractIntroduction Reperfusion surgery following testicular ischemia is a reproductive health threatening status and may result with organ dysfunction in men. The high level of reactive oxygen species (ROS) and cease of blood flow to the testis are the most important reasons of this testicular injury. Until today, numerous experimental studies reported that antioxidants might be efficient to alleviate oxidative stress induced organ dysfunction. For this purpose, in this study, we have investigated the protective effects of xanthine oxidase (XO) inhibitor, allopurinol, and ROS scavenger, trolox, in a comparative perspective in testicular ischemia reperfusion injury subjected rats. Materials and methods Twenty-eight adult male Sprague Dawley rats were divided into four groups of seven animals in each; control, ischemia/reperfusion (I/R), allopurinol and trolox. The rats in control group did not receive any application. Animals in I/R, allopurinol and trolox groups were subjected to 2 h testicular reperfusion injury following 5 h ischemia. Intraperitoneally (i.p.) 1 ml isotonic, 200 mg/kg allopurinol and 50 mg/kg trolox were administered to the animals in these groups 30 min prior reperfusion. At the end of experiment, all animals were sacrificed and blood serum malondialdehyde (MDA) levels were measured. Histological sections were obtained from the testis and stained with hematoxylin and eosin (H&E), proliferating cell nuclear antigen (PCNA) and cleaved caspase-3. Apoptotic index was evaluated with TUNEL Assay. Results Severe morphological degenerations, increased serum MDA, cleaved caspase-3 and TUNEL Assay positivity rate, but reduced PCNA positivity rate was observed in ischemia and reperfusion group. Morphological degenerations, MDA level, apoptotic index and PCNA positive cell rate were slightly alleviated in allopurinol administered animals compared with ischemia and reperfusion group. Protection with trolox was more successful and the results of the analysis were similar to the control group. Discussion Ischemia that leading to testicular torsion is a reproductive health affecting problem and current surgical treatment methods might be insufficient to recover testis. Various types of ROS generating mechanisms in cell are limiting protective potency of allopurinol, and cocktail administration of different ROS inhibitors might be more effective. However, our results indicate that free radical scavenger trolox might be a candidate drug to alleviate degenerative effects of testicular ischemia reperfusion injury. Conclusions This is the first study that demonstrates antioxidant trolox was more successful than XO inhibitor allopurinol to protect testis against ischemia and reperfusion injury in rats. [GRAPHICS]en_US
dc.description.sponsorshipDicle University, Turkey Scientific Research Projects Coordination Unit [Tip.18.01]en_US
dc.description.sponsorshipThis work was financially supported by the Dicle University, Turkey Scientific Research Projects Coordination Unit [Tip.18.01]. Part of the study was oral presented at International Health Science Conference (IHSC-2019), 6e8 November 2019, Diyarbakir, Turkey.en_US
dc.identifier.doi10.1016/j.jpurol.2020.05.008
dc.identifier.issn1477-5131
dc.identifier.issn1873-4898
dc.identifier.issue4en_US
dc.identifier.pmid32595071
dc.identifier.scopus2-s2.0-85086932640
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1016/j.jpurol.2020.05.008
dc.identifier.urihttps://hdl.handle.net/11468/15771
dc.identifier.volume16en_US
dc.identifier.wosWOS:000574920700032
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal of Pediatric Urology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectTestisen_US
dc.subjectIschemia/Reperfusionen_US
dc.subjectApoptosisen_US
dc.subjectAllopurinolen_US
dc.subjectTroloxen_US
dc.titleTrolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in ratsen_US
dc.titleTrolox is more successful than allopurinol to reduce degenerative effects of testicular ischemia/reperfusion injury in rats
dc.typeArticleen_US

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