Estimation of the potential fertility based upon non-return rates of bulls

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dc.contributor.authorTas, M.
dc.contributor.authorBacinoglu, S.
dc.contributor.authorCirit, Ue.
dc.contributor.authorOezguemues, S.
dc.contributor.authorKasgoez, H.
dc.contributor.authorPabuccuoglu, S.
dc.date.accessioned2024-04-24T16:18:28Z
dc.date.available2024-04-24T16:18:28Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn the present study, we aimed to develop a polyacrylamide gel that could be used instead of bovine cervical mucus in the cervical mucus penetration test (CMPT) to obtain coherent and replicable results in bulls. The frozen semen samples of six Holstein bulls, which were divided into two fertility groups as low and high according to their non-return rate (NRR), were used. In this study, the modified CMPT (mCMPT) was carried out within 0.25 mL transparent plastic straws with an inner diameter 1.7 mm. The penetration ability of spermatozoa to bovine cervical mucus and to polyacrylamide gels swollen with two different solutions [NaCl (G1) and PBS (G2)] was compared. For the penetration test, the straws filled with cervical mucus and both gels were dipped into thawed semen samples and incubated at 37 T for 15 min. After the incubation, straws were frozen in liquid nitrogen vapour and stored at -20 degrees C. On the evaluation day, the frozen straws were cut at 1.5-1.75 cm (penetration distance range = PDRI), 3.25-3.5 cm (PDR2) and 5.0-5.25 cm (PDR3), beginning from open-end of the straws. The separated frozen parts were then immediately transferred onto special counting slides by pushing with a mandrel and left to thaw. Thawed samples were covered with cover glass and penetrated spermatozoa in these parts were counted. The relation between the results and fertility of bulls was determined. In the tests performed using mucus, the number of spermatozoa determined in the high fertility group was found to be higher at PDR3 (p < 0.0001) compared to the low fertility group, while in G1 spermatozoa number was significantly higher at PDRI and PDR3 (p < 0.0001). However, in G2 medium, no significant difference was observed between either of the fertility groups with respect to spermatozoa number determined at all distance ranges. In the study, we have determined that the gel swollen with NaCl produces better results and this gel can be used instead of bovine cervical mucus for the CMPT. Therefore, we have concluded that the penetration test performed by polyacrylamide gel swollen with NaCl can be a suitable technique for estimation of the potential fertility of bull spermatozoa. (C) 2007 Elsevier Inc. All rights reserved.en_US
dc.identifier.doi10.1016/j.theriogenology.2007.07.011
dc.identifier.endpage987en_US
dc.identifier.issn0093-691X
dc.identifier.issn1879-3231
dc.identifier.issue7en_US
dc.identifier.pmid17850857
dc.identifier.scopus2-s2.0-34548655283
dc.identifier.scopusqualityQ1
dc.identifier.startpage981en_US
dc.identifier.urihttps://doi.org/10.1016/j.theriogenology.2007.07.011
dc.identifier.urihttps://hdl.handle.net/11468/16118
dc.identifier.volume68en_US
dc.identifier.wosWOS:000249960800005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Incen_US
dc.relation.ispartofTheriogenology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectBull Semenen_US
dc.subjectCervical Mucusen_US
dc.subjectPolyacrylamide Gelen_US
dc.subjectPenetration Testen_US
dc.titleEstimation of the potential fertility based upon non-return rates of bullsen_US
dc.titleEstimation of the potential fertility based upon non-return rates of bulls
dc.typeArticleen_US

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