Effects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liver

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dc.contributor.authorSeker, Ugur
dc.contributor.authorKaya, Seval
dc.contributor.authorKandemir, Sevgi Irtegun
dc.contributor.authorSener, Dila
dc.contributor.authorDemirel, Ozlem Unay
dc.contributor.authorNergiz, Yusuf
dc.date.accessioned2024-04-24T17:17:58Z
dc.date.available2024-04-24T17:17:58Z
dc.date.issued2022
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground and Aim: This study examined the effects of black cumin seed oil treatment on oxidative stress and the expression of radixin and moesin in the liver of experimental diabetic rats. Materials and Methods: Eighteen rats were divided into 3 equal groups (control, diabetes, treatment). The control group was not exposed to any experimental treatment. Streptozotocin was administered to the rats in the diabetes and treatment groups. A 2.5 mL/kg dose of black cumin seed oil was administered daily for 56 days to the treatment group. At the conclusion of the experiment, the blood level of malondialdehyde (MDA) and glutathione (GSH) was measured. The expression level and the cellular distribution of radixin and moesin in the liver were analyzed. Results: The plasma MDA (3.05 +/- 0.45 nmol/mL) and GSH (78.49 +/- 20.45 mu mol/L) levels in the diabetes group were significantly different (p<0.01) from the levels observed in the control group (MDA: 1.09 +/- 0.31 nmol/mL, GSH: 277.29 +/- 17.02 mu mol/L) and the treatment group (MDA: 1.40 +/- 0.53 nmol/mL, GSH: 132.22 +/- 11.81 mu mol/L). Immunohistochemistry and western blotting analyses indicated that while the level of radixin was not significantly between the groups (p>0.05) and moesin expression was significantly downregulated (p<0.05) in the experimental group, the treatment was ineffective. Conclusion: The administered dose was sufficient to prevent oxidative stress, but was not sufficient to alleviate the effects of diabetes on moesin expression in hepatic sinusoidal cells.en_US
dc.description.sponsorshipScientific Research Projects Coordinatorship of Dicle University [TIP.19.018]en_US
dc.description.sponsorshipThis research was partially supported by Scientific Research Projects Coordinatorship of Dicle University (grant no: TIP.19.018).en_US
dc.identifier.doi10.14744/hf.2021.2021.0035
dc.identifier.endpage26en_US
dc.identifier.issn1307-5888
dc.identifier.issn2757-7392
dc.identifier.issue1en_US
dc.identifier.pmid35782372
dc.identifier.startpage21en_US
dc.identifier.trdizinid1170038
dc.identifier.urihttps://doi.org/10.14744/hf.2021.2021.0035
dc.identifier.urihttps://search.trdizin.gov.tr/yayin/detay/1170038
dc.identifier.urihttps://hdl.handle.net/11468/18488
dc.identifier.volume3en_US
dc.identifier.wosWOS:000863555800005
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherKare Publen_US
dc.relation.ispartofHepatology Forum
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiabetesen_US
dc.subjectLiveren_US
dc.subjectMoesinen_US
dc.subjectRadixinen_US
dc.subjectRaten_US
dc.titleEffects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liveren_US
dc.titleEffects of black cumin seed oil on oxidative stress and expression of membrane-cytoskeleton linker proteins, radixin, and moesin in streptozotocin-induced diabetic rat liver
dc.typeArticleen_US

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