The effect of ethanol extract of Hypericum lysimachioides on lipid profile in hypercholesterolemic rabbits and its in vitro antioxidant activity

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dc.contributor.authorHakimoglu, Fidan
dc.contributor.authorKizil, Goksel
dc.contributor.authorKanay, Zeki
dc.contributor.authorKizil, Murat
dc.contributor.authorIsi, Hilmi
dc.date.accessioned2024-04-24T16:10:53Z
dc.date.available2024-04-24T16:10:53Z
dc.date.issued2007
dc.departmentDicle Üniversitesien_US
dc.description.abstractHypercholesterolemia, high cholesterol diet and oxidative stress increase serum total cholesterol and LDL cholesterol levels resulting in increased risk for development of atherosclerosis. Antioxidants play an important role in inhibiting and scavenging radicals, thus providing protection to humans against infectious and degenerative diseases. Literature shows that the antioxidant activity is high in medicinal plants. Realizing the fact that, this study was carried out to determine the effect of ethanol extract of Hypericum lysimachioides Boiss var lysimachioides (Guttifera) on serum lipid levels and serum lipid peroxidation in hypercholesterolemic rabbits. The rabbits were divided into four groups and these groups were fed with diets containing standard laboratory diet (Group 1), standard laboratory diet and ethanol extracts of H. lysimachioides (HL) (50 mg/kg body weight) (Group 11), standard laboratory diet, ethanol extracts of HL (50 mg/kg body weight) and cholesterol (100 mg/kg body weight) (Group 111), and finally standard laboratory diet and cholesterol (100 mg/kg body weight) (Group IV), for 5 weeks. Feeding cholesterol increased serum cholesterol and LDL cholesterol levels significantly in Group IV as compared to the other groups. Ethanol extract of HL with high cholesterol diet significantly lowered LDL cholesterol and total cholesterol levels in the rabbits of Group III as compared to the Group IV. The level of serum triacylglycerol was found to be similar to all comparison groups. HDL cholesterol levels were also increased significantly in Groups 11 and III as compared to Group IV. Statistically significant difference was found in Group IV as compared to all other groups. The ethanol extract of HL with high cholesterol diet significantly lowered the serum MDA levels in the rabbits of Group III compared to the Group IV. The histopathological findings confirmed that the ethanol extract of HL restrained the progression of the hydropic degeneration and fatty changes in the liver and some atherosclerotic lesions in the aorta. The in vitro antioxidant activities of ethanol extract of HL was also evaluated. The free radical-scavenging properties of HL (IC50 = 28 mu g/ml) were studied using 1,1-diphenyl-2-picrylhydrazyi (DPPH) assay system. Since plant phenolic compound is one of the phytochemicals possessing radical scavenging activity, the amount of total phenolic compound was also determined in ethanol extract of HL and total phenolic content of one-milligram HL ethanol extract was equivalent to 307 mu g of gallic acid. Total antioxidant activity of ethanol extract of HL was tested by using ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods. Antioxidative activities of ethanol extract of HL was found to be comparable with Vitamin E. In conclusion, the use of this extract could be useful in the management of cardiovascular disease in which atherosclerosis is important. (c) 2006 Elsevier Ireland Ltd. All rights reserved.en_US
dc.identifier.doi10.1016/j.atherosclerosis.2006.07.013
dc.identifier.endpage122en_US
dc.identifier.issn0021-9150
dc.identifier.issn1879-1484
dc.identifier.issue1en_US
dc.identifier.pmid16901489
dc.identifier.scopus2-s2.0-34247120184
dc.identifier.scopusqualityQ1
dc.identifier.startpage113en_US
dc.identifier.urihttps://doi.org/10.1016/j.atherosclerosis.2006.07.013
dc.identifier.urihttps://hdl.handle.net/11468/15170
dc.identifier.volume192en_US
dc.identifier.wosWOS:000246655400015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Ireland Ltden_US
dc.relation.ispartofAtherosclerosis
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCholesterol Feden_US
dc.subjectLipid Peroxidationen_US
dc.subjectHypericum Lysimachioidesen_US
dc.subjectHypercholesterolemiaen_US
dc.subjectAntioxidant Activityen_US
dc.titleThe effect of ethanol extract of Hypericum lysimachioides on lipid profile in hypercholesterolemic rabbits and its in vitro antioxidant activityen_US
dc.titleThe effect of ethanol extract of Hypericum lysimachioides on lipid profile in hypercholesterolemic rabbits and its in vitro antioxidant activity
dc.typeArticleen_US

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