The obtainment of alkaline serine protease from Bacillus sp. isolated from solid through the solid-state fermentation technique by using rind of the citrullus lanatus L. (watermelon) and cucumis melo L. (melon)
Yükleniyor...
Tarih
2007
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Dicle Üniversitesi Ziya Gökalp Eğitim Fakültesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalışmada, ekonomik önemi olmayan ve birikerek çevre kirliliğine yol açan
karpuz ve kavun kabuğu katı substrat olarak kullanılarak Katı-Faz
Fermentasyonu (SSF) yöntemi ile Bacillus sp.’den yüksek miktarda alkalin
serin proteaz eldesi amaçlandı.
Enzim aktivitesi üzerine pH, sıcaklık, metaller, azot ve karbon kaynakları etkisi
incelendi. Yapılan inhibisyon çalışmasında PMSF ile inhibe olan enzimin
alkalin serin proteaz olduğu tespit edildi.
SSF yöntemi ekstrasellular enzimlerin düşük masrafla, hızlı bir şekilde, bol
miktarda ve çevreye zarar vermeden üretilebildiği bir yöntem olduğu için
kullanıldı.
In this study it was aimed to obtain serine alkaline protease in high amount from Bacillus sp. through solid-phase fermentation method (SSF) by using watermelon and melon rinds of no economic importance, and cause environment pollution by accumulation, as solid substrate. The effects of pH, temperature, metals, nitrogen and carbon sources were examined. In this inhibition study it was determined that the ezyme which is inhibited with PMSF (phenylmethylsulfonyl fluoride) was alkaline serine protease. SSF method was used since extracellular enzymes could be produced at low cost, in a quick way, in plenty and without giving any harm to the environment.
In this study it was aimed to obtain serine alkaline protease in high amount from Bacillus sp. through solid-phase fermentation method (SSF) by using watermelon and melon rinds of no economic importance, and cause environment pollution by accumulation, as solid substrate. The effects of pH, temperature, metals, nitrogen and carbon sources were examined. In this inhibition study it was determined that the ezyme which is inhibited with PMSF (phenylmethylsulfonyl fluoride) was alkaline serine protease. SSF method was used since extracellular enzymes could be produced at low cost, in a quick way, in plenty and without giving any harm to the environment.
Açıklama
Anahtar Kelimeler
Bacillus sp., SSF, Alkalin serin proteaz, Kavun, Karpuz, Alkaline serine protease, Melon, Watermelon
Kaynak
Dicle Üniversitesi Ziya Gökalp Eğitim Fakültesi Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
9
Künye
Fincan, S. A. ve Okumuş, V. (2007). The obtainment of alkaline serine protease from Bacillus sp. isolated from solid through the solid-state fermentation technique by using rind of the citrullus lanatus L. (watermelon) and cucumis melo L. (melon). Dicle Üniversitesi Ziya Gökalp Eğitim Fakültesi Dergisi, (9), 104-114.
