Leishmaniasis in Turkey: Visceral and cutaneous leishmaniasis caused by Leishmania donovani in Turkey

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dc.contributor.authorOzbilgin, Ahmet
dc.contributor.authorHarman, Mehmet
dc.contributor.authorKarakus, Mehmet
dc.contributor.authorBart, Aldert
dc.contributor.authorToz, Seray
dc.contributor.authorKurt, Ozgur
dc.contributor.authorCavus, Ibrahim
dc.date.accessioned2024-04-24T16:10:42Z
dc.date.available2024-04-24T16:10:42Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractIn Turkey, the main causative agents are Leishmania tropica (L. tropica) and Leishmania infantum (L. infant:tun) for cutaneous leishmaniasis (CL) and L. infantum for visceral leishmaniasis (VL). In this study, we investigated leishmaniasis cases caused by L. donovani and established animal models for understanding its tropism in in vivo conditions. Clinical samples (lesion aspirations and bone marrow) obtained from CL/VL patients were investigated using parasitological (smear/NNN) and DNA-based techniques. For species identification, a real time ITS1-PCR was performed using isolates and results were confirmed by hsp70 PCR-N/sequencing and cpb gene PCR/sequencing in order to reveal Leishmania donovani and Leishmania infantum discrimination. Clinical materials from CL and VL patients were also inoculated into two experimental groups (Group CL and Group VL) of Balb/C mice intraperitoneally for creating clinical picture of Turkish L. donovani strains. After 45 days, the samples from visible sores of the skin were taken, and spleens and livers were removed. Measurements of the internal organs were done and touch preparations were prepared for checking the presence of amastigotes. The strains were isolated from all patients and amastigotes were seen in all smears of the patients, and then isolates were immediately stored in liquid nitrogen. In real time ITS1-PCR, the melting temperatures of all samples were out of range of L. infcrnuttn, L. tropica and L. major. Sequencing of hsp70 PCR-N showed that all isolates highly identical to previously submitted L. donovani sequences in GenBank, and cpb gene sequencing showed five isolates had longer cpbF allele, whereas one isolate contained a mixed sequence of both cpbF and cpbE. All mice in both experimental groups became infected. Compared to controls, the length and width of both liver and spleen were significantly elevated (p < 0.001) in both groups of mice. However, the weight of the liver increased significantly in all mice whereas the weight of spleen increased only in VL group. Amastigotes were also seen in all touch preparations prepared from skin sores, spleen and liver. L. donovani strain was isolated from autocutaneous a VL patient first time in Turkey. Animal models using clinical samples were successfully established and important clinical differences of the isolated strains were observed.en_US
dc.description.sponsorshipResearch Funds of Celal Bayar University [CBUBAP 2013-03]en_US
dc.description.sponsorshipThis work was supported by The Research Funds of Celal Bayar University with the Project no: CBUBAP 2013-03. The authors would like to thank to Asc. Prof. Dr. M. Kirami olgen for preparing the map; and to Parasite Bank in Celal Bayar University Faculty of Medicine for keeping and supplying the isolates.en_US
dc.identifier.doi10.1016/j.actatropica.2017.05.032
dc.identifier.endpage96en_US
dc.identifier.issn0001-706X
dc.identifier.issn1873-6254
dc.identifier.pmid28587839
dc.identifier.scopus2-s2.0-85020448363
dc.identifier.scopusqualityQ1
dc.identifier.startpage90en_US
dc.identifier.urihttps://doi.org/10.1016/j.actatropica.2017.05.032
dc.identifier.urihttps://hdl.handle.net/11468/15054
dc.identifier.volume173en_US
dc.identifier.wosWOS:000406571000013
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofActa Tropica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectLeishmaniasisen_US
dc.subjectCutaneousen_US
dc.subjectVisceralen_US
dc.subjectLeishmania Donovanien_US
dc.subjectTurkeyen_US
dc.titleLeishmaniasis in Turkey: Visceral and cutaneous leishmaniasis caused by Leishmania donovani in Turkeyen_US
dc.titleLeishmaniasis in Turkey: Visceral and cutaneous leishmaniasis caused by Leishmania donovani in Turkey
dc.typeArticleen_US

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