The effect of iloprost and sildenafil, alone and in combination, on myocardial ischaemia and nitric oxide and irisin levels

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dc.contributor.authorAydin, Suna
dc.contributor.authorKuloglu, Tuncay
dc.contributor.authorAydin, Suleyman
dc.contributor.authorYardim, Meltem
dc.contributor.authorAzboy, Davut
dc.contributor.authorTemizturk, Zeki
dc.contributor.authorKalkan, Ali Kemal
dc.date.accessioned2024-04-24T17:28:06Z
dc.date.available2024-04-24T17:28:06Z
dc.date.issued2017
dc.departmentDicle Üniversitesien_US
dc.description.abstractAim: Insufficient oxygen supply to organs and tissues due to reduced arterial or venous blood flow results in ischaemia, during which, although ATP production stops, AMP and adenosine continue to be produced from ATP. The fate of irisin, which causes the production of heat instead of ATP during ischaemia, is unknown. Iloprost and sildenafil are two pharmaceutical agents that mediate the resumption of reperfusion (blood supply) via vasodilatation during ischaemic conditions. Our study aimed to explore the effects of iloprost and sildenafil on irisin levels in the heart, liver and kidney tissues and whether these pharmaceutical agents had any impact on serum irisin and nitric oxide levels in rats with induced experimental myocardial ischaemia. Methods: The study included adult male Sprague-Dawley rats aged 10 months and weighing between 250 and 280 g. The animals were randomly allocated to eight groups, with five rats in each group. The groups were: sham (control), ioprost (ILO), sildenafil (SIL), ILO + SIL, myocardial ischaernia (MI), MI + ILO, MI + SIL and MI + ILO + SIL. The treatment protocols were implemented before inducing ischaemia, which was done by occluding the left coronary artery with a plastic ligature for 30 minutes. Following the reperfusion procedure, all rats were sacrificed after 24 hours, and their heart, liver and kidney tissues and blood samples were collected for analyses. An immunohistochemical method was used to measure the change in irisin levels, the ELISA method to quantify blood irisin levels, and Griess' assay to determine nitric oxide (NO) levels in the serum and tissue. Myocardial ischaemia was confirmed based on the results of Masson's trichrome staining, as well as levels of troponin and creatine kinase MB. Results: Irisin levels in biological tissue and serum dropped statistically significantly in the ischaemic group (MI), but were restored with ILO and SIL administration. Individual SIL administration was more potently restorative than individual ILO administration or the combined administration of the two agents. NO level, on the other hand, showed the opposite tendency, reaching the highest level in the MI group, and falling with the use of pharmaceutical agents. Conclusions: Individual or combined administration of ILO and SIL reduced myocardial ischaemia and NO levels, and increased irisin levels. Elevated levels of irisin obtained by drug administration could possibly contribute to accelerated wound recovery by local heat production. Sildenafil was more effective than iloprost in eliminating ischaemia and may be the first choice in offsetting the effects of ischaemia in the future.en_US
dc.description.sponsorshipscientific research unit of Firat University [TF-1508]en_US
dc.description.sponsorshipThis project was supported by the protocol number TF-1508 of the scientific research unit of Firat University, to whom we would like to extend out thanks for their support. This study was orally presented at the 14th Congress of the Turkish Society of Cardiovascular Surgery held in Antalya, Turkey, from 3-6 November 2016.en_US
dc.identifier.doi10.5830/CVJA-2017-025
dc.identifier.endpage396en_US
dc.identifier.issn1995-1892
dc.identifier.issn1680-0745
dc.identifier.issue6en_US
dc.identifier.pmid28906529
dc.identifier.scopus2-s2.0-85039735337
dc.identifier.scopusqualityQ3
dc.identifier.startpage389en_US
dc.identifier.urihttps://doi.org/10.5830/CVJA-2017-025
dc.identifier.urihttps://hdl.handle.net/11468/20326
dc.identifier.volume28en_US
dc.identifier.wosWOS:000418296800011
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherClinics Cardive Publ Pty Ltden_US
dc.relation.ispartofCardiovascular Journal of Africa
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectIloprosten_US
dc.subjectSildenafilen_US
dc.subjectNitric Oxideen_US
dc.subjectIrisinen_US
dc.subjectMyocardial Ischaemia-Reperfusionen_US
dc.titleThe effect of iloprost and sildenafil, alone and in combination, on myocardial ischaemia and nitric oxide and irisin levelsen_US
dc.titleThe effect of iloprost and sildenafil, alone and in combination, on myocardial ischaemia and nitric oxide and irisin levels
dc.typeArticleen_US

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