A new experimental rat model of pancreatitis using Ecballium elaterium

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dc.contributor.authorArslan, Serkan
dc.contributor.authorOkur, Mehmet Hanifi
dc.contributor.authorZeytun, Hikmet
dc.contributor.authorBasuguy, Erol
dc.contributor.authorIbiloglu, Ibrahim
dc.contributor.authorTurkoglu, Ahmet
dc.contributor.authorBozdag, Zubeyr
dc.date.accessioned2024-04-24T16:14:56Z
dc.date.available2024-04-24T16:14:56Z
dc.date.issued2015
dc.departmentDicle Üniversitesien_US
dc.description.abstractBackground: We originally aimed to determine the beneficial effects of Ecballium Elaterium (EE) on acute pancreatitis; however, we observed negative effects of EE on the pancreas. Thus, we used EE in rats to generate a new model of pancreatitis, which we compared with other established pancreatitis models. Methods: A total of 32 Wistar albino rats were used. Rats were divided into 4 groups, each of which contained 8 rats. Group 1 (Control), Group 2 (L-Arginine (LA), Group 3 (LA + EE), Group 4 (EE): Twenty-four hours after that serum parameters were analyzed in the collected blood. Blood samples were transported on mice to the Biochemistry Laboratory following cardiac puncture. The levels of amylase, interleukin (IL)-6, interleukin (IL) 1-beta (IL-1 beta), malondialdhyde (MDA), tumor necrosis factor (TNF)-alpha, total antioxidant status (TAS), and total oxidant status (TOS) were analyzed. Histopathological analysis: The pancreas and lung tissue samples obtained from the rats. Edema, inflammation, vacuolization, and necrosis of the pancreas were assessed using a scoring system ranging from 0 to 4. Edema, hemorrhage and inflammation of the lung tissue were evaluated using a scoring system ranging from 0 to 3. Results: Histopathological analysis revealed that edema, inflammation, necrosis, and hemorrhage were significantly higher in the LA + EE group than in the control group. Moreover, necrosis was higher in the rats that received LA and EE compared to the rats that received only LA or EE. Increases in inflammatory mediator levels, including IL-6, IL-1 beta, TNF-alpha, MDA, and TOS, were observed in all groups as compared to the control group. Moreover, lower TAS levels were detected in all groups but the control group. The increase in IL-1 beta and TNF-alpha levels and the decrease in TAS were statistically significant in all groups (P < 0.05). Conclusions: EE may be used to create a successful acute pancreatitis (AP) model, resulting in edema, necrosis, hemorrhage, and inflammation of the pancreas. The major advantage of this model is that it does not require laparotomy, and can be implemented with only an intraperitoneal injection (IP). Moreover, EE may be combined with other agents, such as LA, to create severe pancreatitis. Further molecular studies are warranted to determine the underlying effects of EE on the pancreas. (C) 2015 Published by Elsevier Ltd on behalf of IJS Publishing Group Limited.en_US
dc.identifier.doi10.1016/j.ijsu.2015.09.067
dc.identifier.endpage164en_US
dc.identifier.issn1743-9191
dc.identifier.issn1743-9159
dc.identifier.pmid26472695
dc.identifier.scopus2-s2.0-84956648619
dc.identifier.scopusqualityQ1
dc.identifier.startpage160en_US
dc.identifier.urihttps://doi.org/10.1016/j.ijsu.2015.09.067
dc.identifier.urihttps://hdl.handle.net/11468/15529
dc.identifier.volume23en_US
dc.identifier.wosWOS:000366651500028
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInternational Journal of Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEcballium Elateriumen_US
dc.subjectRaten_US
dc.subjectExperimental Studyen_US
dc.subjectPancreasen_US
dc.subjectPancreatitisen_US
dc.titleA new experimental rat model of pancreatitis using Ecballium elateriumen_US
dc.titleA new experimental rat model of pancreatitis using Ecballium elaterium
dc.typeArticleen_US

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