Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals
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Tarih
2007
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Medknow Publications and Media Pvt. Ltd
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Purpose: To study the prevalence of TEM-, SHV- and GES-type ?-lactamases among Escherichia coli and Klebsiella pneumoniae strains having ceftazidime MICs higher than 2 mg/L. Methods: A total of 63 E. coli and 41 K. pneumoniae isolated from five different university hospitals were studied for the existence of TEM-, SHV- and GES-type ?-lactamases. Susceptibility tests were carried out according to the criteria of National Committee for Clinical Laboratory Standards. MICs were obtained by agar dilution method. Existence of extended-spectrum ?-lactamases (ESBLs) were assessed by double-disc synergy test (DDST). Existence of the above-mentioned ?-lactamase genes were studied both by PCR with specific oligonucleotide primers and isoelectric focusing methods. Results: None of the isolates were carbapenem-resistant. DDSTs were positive in 50 (79.3%) and 33 (80.5%) of E. coli and K. pneumoniae , respectively. TEM gene was detected in 41 (65.1%) and 19 (46.3%), whereas SHV gene in 18 (28.6%) and 20 (48.8%) of E. coli and K. pneumoniae strains, respectively. GES genes were not detected. Conclusions: TEM and SHV genes are highly prevalent among ESBL-producing E. coli and K. pneumoniae , whereas GES-type ESBLs are absent and found not to be responsible of ceftazidime resistance in Turkish hospitals.
Açıklama
Anahtar Kelimeler
Ceftazidime-Resistant, E. Coli, Esbl, Ges, Klebsiella, Shv, Tem
Kaynak
Indian Journal of Medical Microbiology
WoS Q Değeri
Scopus Q Değeri
Q2
Cilt
25
Sayı
4
Künye
Hoşoǧlu, S., Gündeş, S. G., Kolaylı, F., Karadenizli, A. Y., Demirdaǧ, K., Günaydın, M. ve diğerleri. (2007). Extended-spectrum beta-lactamases in ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae isolates in Turkish hospitals. Indian Journal of Medical Microbiology, 25(4), 346-350.
