Toxicity of the dithiocarbamate fungicide mancozeb to the nontarget soil nematode, Caenorhabditis elegans

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dc.contributor.authorEaston, Anna
dc.contributor.authorGüven, Kemal
dc.contributor.authorde Pomerai, David I.
dc.contributor.orcid0000-0002-0181-3746
dc.date.accessioned2024-04-24T15:59:17Z
dc.date.available2024-04-24T15:59:17Z
dc.date.issued2001
dc.departmentDicle Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.description.abstractWe have previously shown that the dithiocarbamate fungicide, Mancozeb, strongly induces lacZ reporter expression from an endogenous heat-shock promoter (hsp16) in the PC72 transgenic strain of the nematode Caenorhabditis elegans. Such evidence of organismal stress, in a nontarget species at subapplication concentrations, was much less apparent for the related fungicide, Maneb, which only weakly induced reporter expression. We now show that reporter induction by Mancozeb is marginal (<60%) after a few hours' exposure, but increases substantially (to almost 10-fold) after overnight exposure. In conjunction with our previous results using intermediate exposure periods, this suggests that the factor limiting reporter responses is likely to be a slow rate of uptake and/or metabolism of the fungicide. We confirm that a potentially toxic metabolite of dithiocarbamate fungicides, namely ethylenethiourea (ETU), has minimal toxicity toward C. elegans, even after prolonged exposure at high concentrations. We demonstrate that exposure to Mancozeb (but not ETU) significantly inhibits larval growth in C. elegans, although this parameter is not markedly more sensitive than reporter induction as a toxicological endpoint. Finally, we have used two-dimensional electrophoresis to show that high concentrations of both Maneb and Mancozeb drastically simplify the protein spot profile compared with controls. However, only in the latter case is there evidence of novel proteins being induced. Both fungicides appear toxic to C. elegans, but only Mancozeb induces a strong heat-shock response. (C) 2001 John Wiley & Sons, Inc.en_US
dc.identifier.citationEaston, A., Güven, K. ve de Pomerai, D. I. (2001). Toxicity of the dithiocarbamate fungicide Mancozeb to the nontarget soil nematode, Caenorhabditis elegans. Journal of Biochemical and Molecular Toxicology, 15(1), 15-25.
dc.identifier.doi10.1002/1099-0461(2001)15:1<15
dc.identifier.endpage25en_US
dc.identifier.issn1095-6670
dc.identifier.issn1099-0461
dc.identifier.issue1en_US
dc.identifier.pmid11170311
dc.identifier.scopus2-s2.0-0035142786
dc.identifier.scopusqualityQ2
dc.identifier.startpage15en_US
dc.identifier.urihttps://doi.org/10.1002/1099-0461(2001)15:1<15
dc.identifier.urihttps://hdl.handle.net/11468/13943
dc.identifier.urihttps://onlinelibrary.wiley.com/doi/epdf/10.1002/1099-0461%282001%2915%3A1%3C15%3A%3AAID-JBT2%3E3.0.CO%3B2-Z
dc.identifier.volume15en_US
dc.identifier.wosWOS:000166478600002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthorGüven, Kemal
dc.language.isoenen_US
dc.publisherWileyen_US
dc.relation.ispartofJournal of Biochemical and Molecular Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectToxicityen_US
dc.subjectTransgenicen_US
dc.subjectDithiocarbamate fungicideen_US
dc.subjectStress-inducibleen_US
dc.subjectHeat shock responseen_US
dc.subjectLarval growthen_US
dc.titleToxicity of the dithiocarbamate fungicide mancozeb to the nontarget soil nematode, Caenorhabditis elegansen_US
dc.titleToxicity of the dithiocarbamate fungicide mancozeb to the nontarget soil nematode, Caenorhabditis elegans
dc.typeArticleen_US

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